Anti-U5 small nuclear ribonucleoprotein (snRNP) antibodies: A rare anti-U snRNP specificity

Yutaka Okano, Ira N. Targoff, Chester V. Oddis, Takao Fujii, Masataka Kuwana, Kensei Tsuzaka, Michito Hirakata, Tsuneyo Mimori, Joe Craft, Thomas A. Medsger

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Abstract

We surveyed for autoantibodies to the U small nuclear ribonucleoproteins (snRNPs) in sera from 1171 patients with various connective tissue diseases by immunoprecipitation assay. We found serum, termed LaJ, which principally recognized the U5 snRNP from one patient with systemic sclerosis-polymyositis overlap syndrome. Anti-LaJ serum immunoprecipitated predominantly U5 snRNA from a 32PO4-labeled HeLa cell extract and at least five U5-specific proteins as well as the Sm core proteins from a [35S]methionine-labeled extract. Anti-LaJ serum immunoprecipitated both U5 snRNA and these five proteins in identical fractions at 15-20S by parallel sucrose gradient, suggesting physical association of these two components. Thus, we concluded that anti-LaJ serum contained antibody specific to the U5 snRNP. Antibodies specific to U5 snRNP were found in a single serum; in contrast, the prevalence of anti-U1 snRNP and anti-Sm antibody was considerably higher. Thus, the specific proteins of the U5 snRNB are rare targets for autoantibodies in patients with connective tissue disease.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalClinical Immunology and Immunopathology
Volume81
Issue number1
DOIs
Publication statusPublished - 1996 Oct

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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    Okano, Y., Targoff, I. N., Oddis, C. V., Fujii, T., Kuwana, M., Tsuzaka, K., Hirakata, M., Mimori, T., Craft, J., & Medsger, T. A. (1996). Anti-U5 small nuclear ribonucleoprotein (snRNP) antibodies: A rare anti-U snRNP specificity. Clinical Immunology and Immunopathology, 81(1), 41-47. https://doi.org/10.1006/clin.1996.0155