Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum

Yasuo Hosaka, Atsushi Saito, Ryo Maeda, Chie Fukaya, Satoru Morikawa, Asako Makino, Kazuyuki Ishihara, Taneaki Nakagawa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective: To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. Design: Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated. Results: In the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration. Conclusion: These results suggest that 30 s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm.

Original languageEnglish
Pages (from-to)364-368
Number of pages5
JournalArchives of Oral Biology
Volume57
Issue number4
DOIs
Publication statusPublished - 2012 Apr

Fingerprint

Fusobacterium nucleatum
Povidone-Iodine
Porphyromonas gingivalis
Biofilms
polycarbonate
Membranes
Agar
Bacteria

Keywords

  • Antibacterial agent
  • Biofilm
  • F. nucleatum
  • P. gingivalis
  • Periodontopathic bacteria
  • Povidone-iodine

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum. / Hosaka, Yasuo; Saito, Atsushi; Maeda, Ryo; Fukaya, Chie; Morikawa, Satoru; Makino, Asako; Ishihara, Kazuyuki; Nakagawa, Taneaki.

In: Archives of Oral Biology, Vol. 57, No. 4, 04.2012, p. 364-368.

Research output: Contribution to journalArticle

Hosaka, Yasuo ; Saito, Atsushi ; Maeda, Ryo ; Fukaya, Chie ; Morikawa, Satoru ; Makino, Asako ; Ishihara, Kazuyuki ; Nakagawa, Taneaki. / Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum. In: Archives of Oral Biology. 2012 ; Vol. 57, No. 4. pp. 364-368.
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