TY - JOUR
T1 - Antibiotic resistance and gyrA mutation affect the efficacy of 10-day sitafloxacin-metronidazole-esomeprazole therapy for Helicobacter pylori in penicillin allergic patients
AU - Mori, Hideki
AU - Suzuki, Hidekazu
AU - Matsuzaki, Juntaro
AU - Masaoka, Tatsuhiro
AU - Kanai, Takanori
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Young Scientists (B) (grant number 26860527, to J.M.), a Grant-in-Aid for Scientific Research B (grant number 16H05291, to H.S.) Scientific Research C (grant number 25460301, to T.M.), and a Grant-in-Aid for challenging Exploratory Research (grant number 26670065, to H.S.) from the Japan Society for the Promotion of Science (JSPS), MEXT-Supported Program for the Strategic Research Foundation at Private Universities (grant number S1411003, to H.S.), the Princess Takamatsu Cancer Research grants (to H.S.), a grant from the Smoking Research Foundation (to H.S.), a grant from Takeda Science Foundation (to J.M.) and Keio Gijuku Academic Development Funds (to J.M. and to H.S.).
Publisher Copyright:
© 2017, © Author(s) 2017.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Background and aim: Helicobacter pylori (H. pylori) eradication regimen has not been standardized for patients with penicillin allergy. We investigated the association between the efficacy of a 10-day sitafloxacin, metronidazole, and esomeprazole triple regimen and antibiotic resistance, in patients with penicillin allergy. Methods: Penicillin-allergic patients infected with H. pylori were enrolled between March 2014 and November 2015. The minimum inhibitory concentrations (MICs) of sitafloxacin and metronidazole, and the gyrA mutation status of the H. pylori strains were determined before treatment. The cut-off points for antimicrobial resistance were defined as 8.0 µg/ml for metronidazole and 0.12 µg/ml for sitafloxacin. The patients received the triple therapy (20 mg esomeprazole, bid; 250 mg metronidazole, bid; and 100 mg sitafloxacin, bid) for 10 days. Successful eradication was evaluated using the [13C] urea breath test or the H. pylori stool antigen test. Results: Fifty-seven patients were analyzed, and the overall eradication rate was 89.5%. The eradication rate in cases of double antibiotic resistance to metronidazole and sitafloxacin was 40.0%, whereas for other combinations of resistance, this was above 90.0%. Finally, the eradication rate of gyrA mutation-negative strains was 96.2%, whereas for gyrA mutation-positive strains, it was 83.9%. Adverse events were reported in 31.6% of cases, all of which were mild and tolerable. Conclusion: Ten days of sitafloxacin and metronidazole triple therapy was safe and highly effective in eradicating H. pylori in penicillin-allergic patients. Double resistance to metronidazole and sitafloxacin was an important predicting factor for eradication failure. However, 10 days of the sitafloxacin and metronidazole triple therapy was highly effective if the strain was susceptible to either sitafloxacin or metronidazole.
AB - Background and aim: Helicobacter pylori (H. pylori) eradication regimen has not been standardized for patients with penicillin allergy. We investigated the association between the efficacy of a 10-day sitafloxacin, metronidazole, and esomeprazole triple regimen and antibiotic resistance, in patients with penicillin allergy. Methods: Penicillin-allergic patients infected with H. pylori were enrolled between March 2014 and November 2015. The minimum inhibitory concentrations (MICs) of sitafloxacin and metronidazole, and the gyrA mutation status of the H. pylori strains were determined before treatment. The cut-off points for antimicrobial resistance were defined as 8.0 µg/ml for metronidazole and 0.12 µg/ml for sitafloxacin. The patients received the triple therapy (20 mg esomeprazole, bid; 250 mg metronidazole, bid; and 100 mg sitafloxacin, bid) for 10 days. Successful eradication was evaluated using the [13C] urea breath test or the H. pylori stool antigen test. Results: Fifty-seven patients were analyzed, and the overall eradication rate was 89.5%. The eradication rate in cases of double antibiotic resistance to metronidazole and sitafloxacin was 40.0%, whereas for other combinations of resistance, this was above 90.0%. Finally, the eradication rate of gyrA mutation-negative strains was 96.2%, whereas for gyrA mutation-positive strains, it was 83.9%. Adverse events were reported in 31.6% of cases, all of which were mild and tolerable. Conclusion: Ten days of sitafloxacin and metronidazole triple therapy was safe and highly effective in eradicating H. pylori in penicillin-allergic patients. Double resistance to metronidazole and sitafloxacin was an important predicting factor for eradication failure. However, 10 days of the sitafloxacin and metronidazole triple therapy was highly effective if the strain was susceptible to either sitafloxacin or metronidazole.
KW - Sitafloxacin
KW - gyrA
KW - metronidazole
KW - penicillin allergy
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UR - http://www.scopus.com/inward/citedby.url?scp=85030257160&partnerID=8YFLogxK
U2 - 10.1177/2050640616688995
DO - 10.1177/2050640616688995
M3 - Article
AN - SCOPUS:85030257160
SN - 2050-6406
VL - 5
SP - 796
EP - 804
JO - United European Gastroenterology Journal
JF - United European Gastroenterology Journal
IS - 6
ER -