Antibiotic susceptibility according to genotype of penicillin-binding protein and macrolide resistance genes, and serotype of Streptococcus pneumoniae isolates from community-acquired pneumonia in children

Naoko Chiba, Reiko Kobayashi, Keiko Hasegawa, Miyuki Morozumi, Eiichi Nakayama, Takeshi Tajima, Satoshi Iwata, Kimiko Ubukata, Hiroko Endo, Reiko Takayanagi, Mika Numata, Shinobu Ishizawa, Keisuke Sunakawa, Tomohiro Oishi, Shigeru Ohnari, Naohisa Kawamura, Haruo Kuroki, Ritsuko Sakai, Masahiko Nitta, Takao Morinobu & 1 others Koichi Shimizu

Research output: Contribution to journalArticle

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Abstract

Objectives: Antibiotic susceptibilities, genes mediating β-lactam and macrolide resistance, and serotypes were analysed for strains of Streptococcus pneumoniae. Methods: A total of 392 strains of S. pneumoniae were isolated from paediatric patients with community-acquired pneumonia between May 2002 and 2004. All strains were classified into six genotype patterns according to the mutations found in the pbp1a, pbp2x and pbp2b genes identified by PCR. These results are represented by adding 'g', indicating genotypic identification. Results: Thirty-nine percent of the isolates showed mutations in either one or two PBP genes (gPISP, where PISP stands for penicillin-intermediate resistant S. pneumoniae) and 52.3% had mutations in three genes (gPRSP, where PRSP stands for penicillin-resistant S. pneumoniae). The majority of the strains had a macrolide resistance gene: mef(A), (30.6%); erm(B), (48.5%); or both mef(A) and erm(B), (7.7%). The most frequent serotypes of these strains were: 6B (23.2%), 23F (17.6%), 19F (17.3%), 14 (10.5%) and 6A (8.2%). Serotypes of the seven-valent conjugate vaccine covered 70.9% of all isolates, and 89.8% of gPRSP. Serotypes of the strains with cefotaxime MICs of ≥2 mg/L were almost all of a vaccine type. Conclusions: The results suggest that introduction of conjugate vaccines into infants and children is necessary for the prevention of pneumococcal infections in Japan.

Original languageEnglish
Pages (from-to)756-760
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume56
Issue number4
DOIs
Publication statusPublished - 2005 Oct
Externally publishedYes

Fingerprint

Penicillin-Binding Proteins
Macrolides
Streptococcus pneumoniae
Pneumonia
Genotype
Anti-Bacterial Agents
Conjugate Vaccines
Genes
Penicillins
Mutation
Pneumococcal Infections
Lactams
Cefotaxime
Japan
Vaccines
Serogroup
Pediatrics
Polymerase Chain Reaction

Keywords

  • Antibiotic resistance genes
  • PBPs
  • Respiratory tract infections
  • S. pneumoniae

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

Cite this

Antibiotic susceptibility according to genotype of penicillin-binding protein and macrolide resistance genes, and serotype of Streptococcus pneumoniae isolates from community-acquired pneumonia in children. / Chiba, Naoko; Kobayashi, Reiko; Hasegawa, Keiko; Morozumi, Miyuki; Nakayama, Eiichi; Tajima, Takeshi; Iwata, Satoshi; Ubukata, Kimiko; Endo, Hiroko; Takayanagi, Reiko; Numata, Mika; Ishizawa, Shinobu; Sunakawa, Keisuke; Oishi, Tomohiro; Ohnari, Shigeru; Kawamura, Naohisa; Kuroki, Haruo; Sakai, Ritsuko; Nitta, Masahiko; Morinobu, Takao; Shimizu, Koichi.

In: Journal of Antimicrobial Chemotherapy, Vol. 56, No. 4, 10.2005, p. 756-760.

Research output: Contribution to journalArticle

Chiba, N, Kobayashi, R, Hasegawa, K, Morozumi, M, Nakayama, E, Tajima, T, Iwata, S, Ubukata, K, Endo, H, Takayanagi, R, Numata, M, Ishizawa, S, Sunakawa, K, Oishi, T, Ohnari, S, Kawamura, N, Kuroki, H, Sakai, R, Nitta, M, Morinobu, T & Shimizu, K 2005, 'Antibiotic susceptibility according to genotype of penicillin-binding protein and macrolide resistance genes, and serotype of Streptococcus pneumoniae isolates from community-acquired pneumonia in children', Journal of Antimicrobial Chemotherapy, vol. 56, no. 4, pp. 756-760. https://doi.org/10.1093/jac/dki302
Chiba, Naoko ; Kobayashi, Reiko ; Hasegawa, Keiko ; Morozumi, Miyuki ; Nakayama, Eiichi ; Tajima, Takeshi ; Iwata, Satoshi ; Ubukata, Kimiko ; Endo, Hiroko ; Takayanagi, Reiko ; Numata, Mika ; Ishizawa, Shinobu ; Sunakawa, Keisuke ; Oishi, Tomohiro ; Ohnari, Shigeru ; Kawamura, Naohisa ; Kuroki, Haruo ; Sakai, Ritsuko ; Nitta, Masahiko ; Morinobu, Takao ; Shimizu, Koichi. / Antibiotic susceptibility according to genotype of penicillin-binding protein and macrolide resistance genes, and serotype of Streptococcus pneumoniae isolates from community-acquired pneumonia in children. In: Journal of Antimicrobial Chemotherapy. 2005 ; Vol. 56, No. 4. pp. 756-760.
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abstract = "Objectives: Antibiotic susceptibilities, genes mediating β-lactam and macrolide resistance, and serotypes were analysed for strains of Streptococcus pneumoniae. Methods: A total of 392 strains of S. pneumoniae were isolated from paediatric patients with community-acquired pneumonia between May 2002 and 2004. All strains were classified into six genotype patterns according to the mutations found in the pbp1a, pbp2x and pbp2b genes identified by PCR. These results are represented by adding 'g', indicating genotypic identification. Results: Thirty-nine percent of the isolates showed mutations in either one or two PBP genes (gPISP, where PISP stands for penicillin-intermediate resistant S. pneumoniae) and 52.3{\%} had mutations in three genes (gPRSP, where PRSP stands for penicillin-resistant S. pneumoniae). The majority of the strains had a macrolide resistance gene: mef(A), (30.6{\%}); erm(B), (48.5{\%}); or both mef(A) and erm(B), (7.7{\%}). The most frequent serotypes of these strains were: 6B (23.2{\%}), 23F (17.6{\%}), 19F (17.3{\%}), 14 (10.5{\%}) and 6A (8.2{\%}). Serotypes of the seven-valent conjugate vaccine covered 70.9{\%} of all isolates, and 89.8{\%} of gPRSP. Serotypes of the strains with cefotaxime MICs of ≥2 mg/L were almost all of a vaccine type. Conclusions: The results suggest that introduction of conjugate vaccines into infants and children is necessary for the prevention of pneumococcal infections in Japan.",
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T1 - Antibiotic susceptibility according to genotype of penicillin-binding protein and macrolide resistance genes, and serotype of Streptococcus pneumoniae isolates from community-acquired pneumonia in children

AU - Chiba, Naoko

AU - Kobayashi, Reiko

AU - Hasegawa, Keiko

AU - Morozumi, Miyuki

AU - Nakayama, Eiichi

AU - Tajima, Takeshi

AU - Iwata, Satoshi

AU - Ubukata, Kimiko

AU - Endo, Hiroko

AU - Takayanagi, Reiko

AU - Numata, Mika

AU - Ishizawa, Shinobu

AU - Sunakawa, Keisuke

AU - Oishi, Tomohiro

AU - Ohnari, Shigeru

AU - Kawamura, Naohisa

AU - Kuroki, Haruo

AU - Sakai, Ritsuko

AU - Nitta, Masahiko

AU - Morinobu, Takao

AU - Shimizu, Koichi

PY - 2005/10

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N2 - Objectives: Antibiotic susceptibilities, genes mediating β-lactam and macrolide resistance, and serotypes were analysed for strains of Streptococcus pneumoniae. Methods: A total of 392 strains of S. pneumoniae were isolated from paediatric patients with community-acquired pneumonia between May 2002 and 2004. All strains were classified into six genotype patterns according to the mutations found in the pbp1a, pbp2x and pbp2b genes identified by PCR. These results are represented by adding 'g', indicating genotypic identification. Results: Thirty-nine percent of the isolates showed mutations in either one or two PBP genes (gPISP, where PISP stands for penicillin-intermediate resistant S. pneumoniae) and 52.3% had mutations in three genes (gPRSP, where PRSP stands for penicillin-resistant S. pneumoniae). The majority of the strains had a macrolide resistance gene: mef(A), (30.6%); erm(B), (48.5%); or both mef(A) and erm(B), (7.7%). The most frequent serotypes of these strains were: 6B (23.2%), 23F (17.6%), 19F (17.3%), 14 (10.5%) and 6A (8.2%). Serotypes of the seven-valent conjugate vaccine covered 70.9% of all isolates, and 89.8% of gPRSP. Serotypes of the strains with cefotaxime MICs of ≥2 mg/L were almost all of a vaccine type. Conclusions: The results suggest that introduction of conjugate vaccines into infants and children is necessary for the prevention of pneumococcal infections in Japan.

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KW - Antibiotic resistance genes

KW - PBPs

KW - Respiratory tract infections

KW - S. pneumoniae

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