Antibodies to different peptides in osteopontin reveal complexities in the various secreted forms

Shigeyuki Kon, Masahiro Maeda, Tatsuya Segawa, Yoshiaki Hagiwara, Yuko Horikoshi, Shunsuke Chikuma, Kumiko Tanaka, Mohammod Mizanur Rashid, Manabu Inobe, Ann F. Chambers, Toshimitsu Uede

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

We have generated synthetic peptides corresponding to various portions of human osteopontin (OPN) and have immunized rabbits and mice with these peptides to generate polyclonal and monoclonal antibodies specific to human OPN. We then generated six distinct sandwich enzyme-linked immunoabsorbent assay (ELISA) systems by using different pairs of polyclonal and monoclonal antibodies against human OPN. These systems allowed us to detect not only various isoforms and truncated forms of recombinant OPN, but also the glycosylated form of native urinary OPN. Most importantly, tumor-derived OPN was differentially detected by the six ELISA systems. The ELISA systems that we have developed will be useful for clarifying the functional roles for OPN in vivo in various physiologic and pathologic conditions. (C) 2000 Wiley- Liss, Inc.

Original languageEnglish
Pages (from-to)487-498
Number of pages12
JournalJournal of Cellular Biochemistry
Volume77
Issue number3
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • ELISA
  • Isoforms
  • Osteopontin
  • Tumor cells
  • Urine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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