Antibody Clicking as a Strategy to Modify Antibody Functionalities on the Surface of Targeted Cells

Toru Komatsu, Etsu Kyo, Haruki Ishii, Kyoji Tsuchikama, Aiko Yamaguchi, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We established a methodology for initiating cross-linking of antibodies selectively on the cell surface through intermolecular copper-free click reactions facilitated by increased effective concentrations of antibodies binding to target antigens. Upon cross-linking of tetrazine- and bicyclononyne-modified trastuzumab on the surface of HER2-overexpressing cells, increased antibody uptake and activation of intracellular signaling were observed. Our findings demonstrate that the cross-linking reaction can significantly alter the biophysical properties of proteins, activating their unique functionalities on targeted cells to realize an increased cargo delivery and synthetic manipulation of cellular signaling.

Original languageEnglish
Pages (from-to)15644-15648
Number of pages5
JournalJournal of the American Chemical Society
Volume142
Issue number37
DOIs
Publication statusPublished - 2020 Sep 16
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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