Antibody to the inositol trisphosphate receptor blocks thimerosalenhanced Ca2+-induced Ca2+ release and Ca2+ oscillations in hamster eggs

Shun ichi Miyazaki, Hideki Shirakawa, Ken Nakada, Yoshiko Honda, Michisuke Yuzaki, Shinji Nakade, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

The sulfhydryl reagent thimerosal enhanced the sensitivity of hamster eggs to injected inositol 1,4,5-trisphosphate (InsP3) or Ca2+ to generate regenerative Ca2+ release from intracellular pools. A monoclonal antibody (mAb) to the InsP3 receptor blocked both the InsP3-induced Ca2+ release (IICR) and Ca2+-induced Ca2+ release (CICR). The mAb also blocked Ca2+ oscillations induced by thimerosal. The results indicate that thimerosal enhances IICR sensitized by cytosolic Ca2+, but not CICR from InsP3-insensitive pools, and causes repetitive Ca2+ releases from InsP3-sensitive pools.

Original languageEnglish
Pages (from-to)180-184
Number of pages5
JournalFEBS Letters
Volume309
Issue number2
DOIs
Publication statusPublished - 1992 Sep 7
Externally publishedYes

Fingerprint

Thimerosal
Inositol
Cricetinae
Eggs
Antibodies
Monoclonal Antibodies
Sulfhydryl Reagents
Inositol 1,4,5-Trisphosphate

Keywords

  • Anti-InsP receptor antibody
  • Ca oscillation
  • Ca-induced Ca release
  • Hamster egg
  • InsP-induced Ca release
  • Sulfhydryl reagent

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Antibody to the inositol trisphosphate receptor blocks thimerosalenhanced Ca2+-induced Ca2+ release and Ca2+ oscillations in hamster eggs. / Miyazaki, Shun ichi; Shirakawa, Hideki; Nakada, Ken; Honda, Yoshiko; Yuzaki, Michisuke; Nakade, Shinji; Mikoshiba, Katsuhiko.

In: FEBS Letters, Vol. 309, No. 2, 07.09.1992, p. 180-184.

Research output: Contribution to journalArticle

Miyazaki, Shun ichi ; Shirakawa, Hideki ; Nakada, Ken ; Honda, Yoshiko ; Yuzaki, Michisuke ; Nakade, Shinji ; Mikoshiba, Katsuhiko. / Antibody to the inositol trisphosphate receptor blocks thimerosalenhanced Ca2+-induced Ca2+ release and Ca2+ oscillations in hamster eggs. In: FEBS Letters. 1992 ; Vol. 309, No. 2. pp. 180-184.
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AU - Honda, Yoshiko

AU - Yuzaki, Michisuke

AU - Nakade, Shinji

AU - Mikoshiba, Katsuhiko

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