Antibody to the inositol trisphosphate receptor blocks thimerosalenhanced Ca2+-induced Ca2+ release and Ca2+ oscillations in hamster eggs

Shun ichi Miyazaki, Hideki Shirakawa, Ken Nakada, Yoshiko Honda, Michisuke Yuzaki, Shinji Nakade, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The sulfhydryl reagent thimerosal enhanced the sensitivity of hamster eggs to injected inositol 1,4,5-trisphosphate (InsP3) or Ca2+ to generate regenerative Ca2+ release from intracellular pools. A monoclonal antibody (mAb) to the InsP3 receptor blocked both the InsP3-induced Ca2+ release (IICR) and Ca2+-induced Ca2+ release (CICR). The mAb also blocked Ca2+ oscillations induced by thimerosal. The results indicate that thimerosal enhances IICR sensitized by cytosolic Ca2+, but not CICR from InsP3-insensitive pools, and causes repetitive Ca2+ releases from InsP3-sensitive pools.

Original languageEnglish
Pages (from-to)180-184
Number of pages5
JournalFEBS Letters
Volume309
Issue number2
DOIs
Publication statusPublished - 1992 Sep 7

Keywords

  • Anti-InsP receptor antibody
  • Ca oscillation
  • Ca-induced Ca release
  • Hamster egg
  • InsP-induced Ca release
  • Sulfhydryl reagent

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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