Anticoagulant factor protein S inhibits the proliferation of rat astrocytes after injury

Yoko I. Tomobe; Hiroshi Hama; Takeshi Sakurai; Akira Fujimori; Yoichiro Abe; Katsutoshi Goto

doi:10.1016/0304-3940(96)12880-9

Anticoagulant factor protein S inhibits the proliferation of rat astrocytes after injury

Yoko I. Tomobe, Hiroshi Hama, Takeshi Sakurai, Akira Fujimori, Yoichiro Abe, Katsutoshi Goto

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The actions of protein S (PS) on the scratch injury-induced proliferation of rat astrocytes (AC) were studied. PS (10-300 nM) markedly inhibited [³H]thymidine incorporation into injured AC. The effect of 100 nM PS was comparable with that of transforming growth factor-β1 (TGF-β1; 20 ng/ml). The incorporation of bromodeoxyuridine, which is usually detectable in AC along the border of the wound, was undetectable in the presence of 300 nM PS. The level of PS mRNA in the injured AC was slightly increased 15 h after the injury, although the level of its receptor, Tyro 3 mRNA was not changed significantly. The results of the present study suggest that PS plays an important role in tissue repair processes in the central nervous system (CNS) by suppressing the proliferation of AC as in the case of TGF-β1.

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Neuroscience Letters
Volume	214
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(96)12880-9
Publication status	Published - 1996 Aug 16
Externally published	Yes

Keywords

Astrocyte
Growth inhibition
Proliferation
Protein S
Transforming growth factor-
Tyro 3

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/0304-3940(96)12880-9

Cite this

@article{918680f3be9645c799335d6f4b73ce37,

title = "Anticoagulant factor protein S inhibits the proliferation of rat astrocytes after injury",

abstract = "The actions of protein S (PS) on the scratch injury-induced proliferation of rat astrocytes (AC) were studied. PS (10-300 nM) markedly inhibited [3H]thymidine incorporation into injured AC. The effect of 100 nM PS was comparable with that of transforming growth factor-β1 (TGF-β1; 20 ng/ml). The incorporation of bromodeoxyuridine, which is usually detectable in AC along the border of the wound, was undetectable in the presence of 300 nM PS. The level of PS mRNA in the injured AC was slightly increased 15 h after the injury, although the level of its receptor, Tyro 3 mRNA was not changed significantly. The results of the present study suggest that PS plays an important role in tissue repair processes in the central nervous system (CNS) by suppressing the proliferation of AC as in the case of TGF-β1.",

keywords = "Astrocyte, Growth inhibition, Proliferation, Protein S, Transforming growth factor-, Tyro 3",

author = "Tomobe, {Yoko I.} and Hiroshi Hama and Takeshi Sakurai and Akira Fujimori and Yoichiro Abe and Katsutoshi Goto",

year = "1996",

month = aug,

day = "16",

doi = "10.1016/0304-3940(96)12880-9",

language = "English",

volume = "214",

pages = "57--60",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Anticoagulant factor protein S inhibits the proliferation of rat astrocytes after injury

AU - Tomobe, Yoko I.

AU - Hama, Hiroshi

AU - Sakurai, Takeshi

AU - Fujimori, Akira

AU - Abe, Yoichiro

AU - Goto, Katsutoshi

PY - 1996/8/16

Y1 - 1996/8/16

N2 - The actions of protein S (PS) on the scratch injury-induced proliferation of rat astrocytes (AC) were studied. PS (10-300 nM) markedly inhibited [3H]thymidine incorporation into injured AC. The effect of 100 nM PS was comparable with that of transforming growth factor-β1 (TGF-β1; 20 ng/ml). The incorporation of bromodeoxyuridine, which is usually detectable in AC along the border of the wound, was undetectable in the presence of 300 nM PS. The level of PS mRNA in the injured AC was slightly increased 15 h after the injury, although the level of its receptor, Tyro 3 mRNA was not changed significantly. The results of the present study suggest that PS plays an important role in tissue repair processes in the central nervous system (CNS) by suppressing the proliferation of AC as in the case of TGF-β1.

AB - The actions of protein S (PS) on the scratch injury-induced proliferation of rat astrocytes (AC) were studied. PS (10-300 nM) markedly inhibited [3H]thymidine incorporation into injured AC. The effect of 100 nM PS was comparable with that of transforming growth factor-β1 (TGF-β1; 20 ng/ml). The incorporation of bromodeoxyuridine, which is usually detectable in AC along the border of the wound, was undetectable in the presence of 300 nM PS. The level of PS mRNA in the injured AC was slightly increased 15 h after the injury, although the level of its receptor, Tyro 3 mRNA was not changed significantly. The results of the present study suggest that PS plays an important role in tissue repair processes in the central nervous system (CNS) by suppressing the proliferation of AC as in the case of TGF-β1.

KW - Astrocyte

KW - Growth inhibition

KW - Proliferation

KW - Protein S

KW - Transforming growth factor-

KW - Tyro 3

UR - http://www.scopus.com/inward/record.url?scp=0030590246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030590246&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(96)12880-9

DO - 10.1016/0304-3940(96)12880-9

M3 - Article

C2 - 8873131

AN - SCOPUS:0030590246

SN - 0304-3940

VL - 214

SP - 57

EP - 60

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1

ER -

Anticoagulant factor protein S inhibits the proliferation of rat astrocytes after injury

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this