Antigen common to several species, recognized by a rat monoclonal antibody raised against syngeneic rat bladder tumor

H. Eto, Hideyuki Saya, M. Nakata, A. Mizoguchi, S. Kamidono

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A rat monoclonal antibody (MAb) termed RS-II (Ig M) was obtained by syngeneic immunization with rat bladder tumor cells induced by N-butyl, N-hydroxybutylnitrosamine (BBN). Immunocytochemical analysis showed that RS-II is also reactive with mouse, dog and human bladder tumor-cell lines and some other human tumor-cell lines but not myeloma or leukemia cells. Immunohistochemical examination of paraffin-embedded tissues has shown that RS-II is reactive with mouse, rat, dog and human bladder tumors (5/5, 5/5, 1/1, 31/49) and some other tumors, but not with normal human urothelium or normal rat tissues. The antigen is expressed on the majority of low-grade or well-differentiated tumors, but less on advanced, invasive tumors. The immunofluorescence staining pattern of cultured cells was cytoplasmic and filamentous. Immunoblotting revealed that the antigen is a M(r) 54,000 and M(r) 56,000 protein which was extracted with difficulty from the cultured cells with Triton X-100. The antigen was also detected in the culture supernatant by means of ELISA. Our results suggest that the epitope is expressed on cytoskeletons common to a wide variety of mammalian cells at a certain stage of differentiation.

Original languageEnglish
Pages (from-to)454-459
Number of pages6
JournalInternational Journal of Cancer
Volume44
Issue number3
Publication statusPublished - 1989
Externally publishedYes

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Urinary Bladder Neoplasms
Monoclonal Antibodies
Antigens
Tumor Cell Line
Cultured Cells
Dogs
Urothelium
Neoplasms
Octoxynol
Cytoskeleton
Immunoblotting
Paraffin
Fluorescent Antibody Technique
Epitopes
Immunization
Leukemia
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Antigen common to several species, recognized by a rat monoclonal antibody raised against syngeneic rat bladder tumor. / Eto, H.; Saya, Hideyuki; Nakata, M.; Mizoguchi, A.; Kamidono, S.

In: International Journal of Cancer, Vol. 44, No. 3, 1989, p. 454-459.

Research output: Contribution to journalArticle

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AU - Saya, Hideyuki

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AU - Kamidono, S.

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