Antigen-independent development of Foxp3 + regulatory T cells suppressing autoantibody production in experimental pemphigus vulgaris

Tomoaki Yokoyama, Satoshi Matsuda, Yujiro Takae, Naoko Wada, Takeji Nishikawa, Masayuki Amagai, Shigeo Koyasu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The CD4 +CD25 +Foxp3 + regulatory T cells (Tregs) play suppressive roles in various types of autoimmunity. It has been reported that Tregs develop in the thymus after high-affinity interaction of their TCR with self-peptide/MHC ligands mostly utilizing TCR-transgenic system. In this study, we examined whether the specific antigen is involved in the development of polyclonal Tregs in pemphigus vulgaris (PV), an autoimmune blistering disease caused by anti-desmoglein 3 (Dsg3) IgG antibodies, as a model system. Adoptive transfer of splenocytes of Dsg3 -/- mice immunized with recombinant mouse Dsg3 to Rag2 -/- recipient mice expressing Dsg3 resulted in the stable production of anti-Dsg3 IgG and the development of PV phenotypes. We show here that Tregs control anti-Dsg3 antibody production in PV model mice: the adoptive transfer of Tregs and the depletion of endogenous Tregs suppressed and augmented, respectively, the anti-Dsg3 antibody production. To examine whether the endogenous expression of Dsg3 is involved in the generation of these PV-relevant Tregs, we compared the potential of wild-type Tregs with that of Tregs from Dsg3. -/- mice. Polyclonal Tregs from Dsg3 -/- mice were more potent than that of wild-type mice, in both adoptive transfer and Treg-depletion experiments, while suppressive activities against IgG production against an irrelevant antigen were similar between Tregs from wild-type and Dsg3 -/- mice. Our observation implies that Tregs capable of suppressing T h cells that drive autoantibody production can develop in the absence of the target antigen.

Original languageEnglish
Article numberdxr020
Pages (from-to)365-373
Number of pages9
JournalInternational Immunology
Volume23
Issue number6
DOIs
Publication statusPublished - 2011

Fingerprint

Desmoglein 3
Pemphigus
Regulatory T-Lymphocytes
Autoantibodies
Antigens
Adoptive Transfer
Immunoglobulin G
Antibody Formation
Autoimmunity
Thymus Gland
Autoimmune Diseases

Keywords

  • Autoantibodies
  • Autoimmunity
  • Skin
  • T cells
  • Tolerance/suppression/anergy

ASJC Scopus subject areas

  • Immunology

Cite this

Antigen-independent development of Foxp3 + regulatory T cells suppressing autoantibody production in experimental pemphigus vulgaris. / Yokoyama, Tomoaki; Matsuda, Satoshi; Takae, Yujiro; Wada, Naoko; Nishikawa, Takeji; Amagai, Masayuki; Koyasu, Shigeo.

In: International Immunology, Vol. 23, No. 6, dxr020, 2011, p. 365-373.

Research output: Contribution to journalArticle

Yokoyama, Tomoaki ; Matsuda, Satoshi ; Takae, Yujiro ; Wada, Naoko ; Nishikawa, Takeji ; Amagai, Masayuki ; Koyasu, Shigeo. / Antigen-independent development of Foxp3 + regulatory T cells suppressing autoantibody production in experimental pemphigus vulgaris. In: International Immunology. 2011 ; Vol. 23, No. 6. pp. 365-373.
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