Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders

Shigeto Shimmura, Jun Shimazaki, Yoshie Ohashi, Kazuo Tsubota

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Purpose. To determine whether the sequestration of inflammatory cells plays a role in the antiinflammatory effects of amniotic membrane transplantation to the ocular surface. Methods. Amniotic membrane grafts were prepared from placental tissue procured from mothers undergoing planned Cesarean sections. A detailed explanation was given to all donors, and a written consent was obtained before processing. Amniotic membrane tissue was dissected into 3- × 3-cm segments, rinsed in phosphate buffered saline, and stored in dimethyl sulfoxide solutions at -80°C until use. In a clinical series, amniotic membrane patches of the ocular surface were performed in 20 eyes of 20 patients with persistent corneal epithelial defects, or as a prophylactic measure after corneal limbal transplantation. Amniotic membrane patches were harvested after a 1-week observation period and were subjected to histopathologic examinations by hematoxylin and eosin staining. Inflammatory cells trapped within the amniotic membrane were labeled by immunocytochemistry using anti-CD14, CD4, CD8, and CD20 antibodies. TUNEL (TdT-mediated dUTP nick end labeling) staining was done to observe cells undergoing apoptosis. The T cell line Molt 4 was co-cultured with amniotic membrane in vitro to observe adhesion of T cells to amniotic membrane. Resuits. Various degrees of inflammatory cell infiltration were observed in all clinical samples of amniotic membrane patches. Most of the inflammatory cells stained positively with anti-CD14 antibodies, indicating that these cells were of monocyte/macrophage lineage. Subsets of T cells included both CD4(+) and CD8(+) cells, whereas CD20(+) cells were sparse. TUNEL assays revealed that trapped inflammatory cells exhibited characteristics of cells undergoing apoptosis. Molt 4 invaded within amniotic membrane in an in vitro assay, which was not inhibited by blocking antibodies to β1 and β2 integrins. Conclusion. Amniotic membrane attracts and traps inflammatory cells infiltrating the ocular surface, which may explain some of the antiinflammatory properties of the fetal tissue.

Original languageEnglish
Pages (from-to)408-413
Number of pages6
JournalCornea
Volume20
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Amnion
Anti-Inflammatory Agents
Transplantation
Apoptosis
Staining and Labeling
T-Lymphocytes
Blocking Antibodies
Corneal Transplantation
T-Lymphocyte Subsets
Hematoxylin
Eosine Yellowish-(YS)
Dimethyl Sulfoxide
Integrins
Cesarean Section
Monocytes
Anti-Idiotypic Antibodies
Fetus
Immunohistochemistry
Macrophages
Phosphates

Keywords

  • Amniotic membrane
  • Inflammation
  • Lymphocytes
  • Ocular surface reconstruction
  • Polymorphonucleocytes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders. / Shimmura, Shigeto; Shimazaki, Jun; Ohashi, Yoshie; Tsubota, Kazuo.

In: Cornea, Vol. 20, No. 4, 2001, p. 408-413.

Research output: Contribution to journalArticle

@article{f8989deed0ec40869e8167bd3f4cc615,
title = "Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders",
abstract = "Purpose. To determine whether the sequestration of inflammatory cells plays a role in the antiinflammatory effects of amniotic membrane transplantation to the ocular surface. Methods. Amniotic membrane grafts were prepared from placental tissue procured from mothers undergoing planned Cesarean sections. A detailed explanation was given to all donors, and a written consent was obtained before processing. Amniotic membrane tissue was dissected into 3- × 3-cm segments, rinsed in phosphate buffered saline, and stored in dimethyl sulfoxide solutions at -80°C until use. In a clinical series, amniotic membrane patches of the ocular surface were performed in 20 eyes of 20 patients with persistent corneal epithelial defects, or as a prophylactic measure after corneal limbal transplantation. Amniotic membrane patches were harvested after a 1-week observation period and were subjected to histopathologic examinations by hematoxylin and eosin staining. Inflammatory cells trapped within the amniotic membrane were labeled by immunocytochemistry using anti-CD14, CD4, CD8, and CD20 antibodies. TUNEL (TdT-mediated dUTP nick end labeling) staining was done to observe cells undergoing apoptosis. The T cell line Molt 4 was co-cultured with amniotic membrane in vitro to observe adhesion of T cells to amniotic membrane. Resuits. Various degrees of inflammatory cell infiltration were observed in all clinical samples of amniotic membrane patches. Most of the inflammatory cells stained positively with anti-CD14 antibodies, indicating that these cells were of monocyte/macrophage lineage. Subsets of T cells included both CD4(+) and CD8(+) cells, whereas CD20(+) cells were sparse. TUNEL assays revealed that trapped inflammatory cells exhibited characteristics of cells undergoing apoptosis. Molt 4 invaded within amniotic membrane in an in vitro assay, which was not inhibited by blocking antibodies to β1 and β2 integrins. Conclusion. Amniotic membrane attracts and traps inflammatory cells infiltrating the ocular surface, which may explain some of the antiinflammatory properties of the fetal tissue.",
keywords = "Amniotic membrane, Inflammation, Lymphocytes, Ocular surface reconstruction, Polymorphonucleocytes",
author = "Shigeto Shimmura and Jun Shimazaki and Yoshie Ohashi and Kazuo Tsubota",
year = "2001",
doi = "10.1097/00003226-200105000-00015",
language = "English",
volume = "20",
pages = "408--413",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders

AU - Shimmura, Shigeto

AU - Shimazaki, Jun

AU - Ohashi, Yoshie

AU - Tsubota, Kazuo

PY - 2001

Y1 - 2001

N2 - Purpose. To determine whether the sequestration of inflammatory cells plays a role in the antiinflammatory effects of amniotic membrane transplantation to the ocular surface. Methods. Amniotic membrane grafts were prepared from placental tissue procured from mothers undergoing planned Cesarean sections. A detailed explanation was given to all donors, and a written consent was obtained before processing. Amniotic membrane tissue was dissected into 3- × 3-cm segments, rinsed in phosphate buffered saline, and stored in dimethyl sulfoxide solutions at -80°C until use. In a clinical series, amniotic membrane patches of the ocular surface were performed in 20 eyes of 20 patients with persistent corneal epithelial defects, or as a prophylactic measure after corneal limbal transplantation. Amniotic membrane patches were harvested after a 1-week observation period and were subjected to histopathologic examinations by hematoxylin and eosin staining. Inflammatory cells trapped within the amniotic membrane were labeled by immunocytochemistry using anti-CD14, CD4, CD8, and CD20 antibodies. TUNEL (TdT-mediated dUTP nick end labeling) staining was done to observe cells undergoing apoptosis. The T cell line Molt 4 was co-cultured with amniotic membrane in vitro to observe adhesion of T cells to amniotic membrane. Resuits. Various degrees of inflammatory cell infiltration were observed in all clinical samples of amniotic membrane patches. Most of the inflammatory cells stained positively with anti-CD14 antibodies, indicating that these cells were of monocyte/macrophage lineage. Subsets of T cells included both CD4(+) and CD8(+) cells, whereas CD20(+) cells were sparse. TUNEL assays revealed that trapped inflammatory cells exhibited characteristics of cells undergoing apoptosis. Molt 4 invaded within amniotic membrane in an in vitro assay, which was not inhibited by blocking antibodies to β1 and β2 integrins. Conclusion. Amniotic membrane attracts and traps inflammatory cells infiltrating the ocular surface, which may explain some of the antiinflammatory properties of the fetal tissue.

AB - Purpose. To determine whether the sequestration of inflammatory cells plays a role in the antiinflammatory effects of amniotic membrane transplantation to the ocular surface. Methods. Amniotic membrane grafts were prepared from placental tissue procured from mothers undergoing planned Cesarean sections. A detailed explanation was given to all donors, and a written consent was obtained before processing. Amniotic membrane tissue was dissected into 3- × 3-cm segments, rinsed in phosphate buffered saline, and stored in dimethyl sulfoxide solutions at -80°C until use. In a clinical series, amniotic membrane patches of the ocular surface were performed in 20 eyes of 20 patients with persistent corneal epithelial defects, or as a prophylactic measure after corneal limbal transplantation. Amniotic membrane patches were harvested after a 1-week observation period and were subjected to histopathologic examinations by hematoxylin and eosin staining. Inflammatory cells trapped within the amniotic membrane were labeled by immunocytochemistry using anti-CD14, CD4, CD8, and CD20 antibodies. TUNEL (TdT-mediated dUTP nick end labeling) staining was done to observe cells undergoing apoptosis. The T cell line Molt 4 was co-cultured with amniotic membrane in vitro to observe adhesion of T cells to amniotic membrane. Resuits. Various degrees of inflammatory cell infiltration were observed in all clinical samples of amniotic membrane patches. Most of the inflammatory cells stained positively with anti-CD14 antibodies, indicating that these cells were of monocyte/macrophage lineage. Subsets of T cells included both CD4(+) and CD8(+) cells, whereas CD20(+) cells were sparse. TUNEL assays revealed that trapped inflammatory cells exhibited characteristics of cells undergoing apoptosis. Molt 4 invaded within amniotic membrane in an in vitro assay, which was not inhibited by blocking antibodies to β1 and β2 integrins. Conclusion. Amniotic membrane attracts and traps inflammatory cells infiltrating the ocular surface, which may explain some of the antiinflammatory properties of the fetal tissue.

KW - Amniotic membrane

KW - Inflammation

KW - Lymphocytes

KW - Ocular surface reconstruction

KW - Polymorphonucleocytes

UR - http://www.scopus.com/inward/record.url?scp=0035032236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035032236&partnerID=8YFLogxK

U2 - 10.1097/00003226-200105000-00015

DO - 10.1097/00003226-200105000-00015

M3 - Article

C2 - 11333331

AN - SCOPUS:0035032236

VL - 20

SP - 408

EP - 413

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 4

ER -