Antiparasitic effect of calcium and magnesium ion-free buffer treatments against a common monogenean Neobenedenia girellae

H. Ohashi, N. Umeda, N. Hirazawa, Y. Ozaki, C. Miura, T. Miura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study investigated a new effective method for controlling the capsalid monogenean Neobenedenia girellae. We examined in vitro and in vivo the effect on the percentage survival of N. girellae in buffers containing different metallic ions. Decreased survival was observed in buffer solutions lacking two ions. In particular, the percentage survival of N. girellae was significantly decreased after 10 min exposure to buffer containing neither Ca2+ nor Mg2+. Transmission electron microscopic observations showed that treatment with this buffer disrupted intercellular junctions. This significant effect on percentage survival of N. girellae using Ca2+/Mg 2+-free buffer was confirmed in an in vivo assay. Ca 2+/Mg2+-free buffer had no effect on the condition of the host, spotted halibut Verasper variegates (Pleuronectidae). These results suggest that treatment with Ca2+/Mg2+-free buffer is a new effective control method, which could replace existing control methods.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalParasitology
Volume134
Issue number2
DOIs
Publication statusPublished - 2007 Feb
Externally publishedYes

Fingerprint

Antiparasitic Agents
Magnesium
magnesium
Buffers
buffers
Ions
ions
Calcium
calcium
control methods
Pleuronectidae
intercellular junctions
Flounder
halibut
Intercellular Junctions
electrons
Electrons
assays

Keywords

  • Calcium ion
  • Cell junction
  • Magnesium ion
  • Monogenea
  • Neobenedenia girellae

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Antiparasitic effect of calcium and magnesium ion-free buffer treatments against a common monogenean Neobenedenia girellae. / Ohashi, H.; Umeda, N.; Hirazawa, N.; Ozaki, Y.; Miura, C.; Miura, T.

In: Parasitology, Vol. 134, No. 2, 02.2007, p. 229-236.

Research output: Contribution to journalArticle

Ohashi, H. ; Umeda, N. ; Hirazawa, N. ; Ozaki, Y. ; Miura, C. ; Miura, T. / Antiparasitic effect of calcium and magnesium ion-free buffer treatments against a common monogenean Neobenedenia girellae. In: Parasitology. 2007 ; Vol. 134, No. 2. pp. 229-236.
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