Antisense oligodeoxynucleotide against c-myc mRNA induces differentiation of human hepatocellular carcinoma cells.

H. Ebinuma, Hidetsugu Saito, Yoshimasa Saito, K. Wakabayashi, M. Nakamura, I. Kurose, H. Ishii

Research output: Contribution to journalArticle

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Abstract

We have previously demonstrated that the human liver-specific antigen (HLSA) expression was enhanced and c-myc levels were reduced during sodium butyrate-induced differentiation in human hepatoma cells. To further elucidate a linkage between the reduction of c-myc levels and an increase in the HLSA expression, antisense oligodeoxynucleotide against c-myc mRNA was transferred into human hepatoma cells. Human hepatoma cell lines, HCC-M, HCC-T and PLC/PRF/5 were transfected with antisense oligodeoxynucleotide and changes in the cell cycle, expression of the HLSA, albumin, and alpha-fetoprotein were examined. Antisense oligodeoxynucleotide was successfully induced into cells visualized by a confocal microscope using fluorescein-labeled oligodeoxynucleotides, and Northern blot analysis revealed that c-myc expression was reduced three and six hours after the transfection. Following these changes, cell proliferation was inhibited and flow cytometric analysis showed that cell number in the G1 phase significantly increased. Increased expression of the HLSA and albumin, and decreased expression of alpha-fetoprotein was observed by flow cytometry in accordance with those changes. These results showed similar changes to those induced by butyrate-treatment obtained in our previous studies. The present study indicates that the reduction of c-myc transcription increases HLSA expression levels through intracellular changes similar to those induced by butyrate, a differentiation inducer.

Original languageEnglish
Pages (from-to)991-999
Number of pages9
JournalInternational Journal of Oncology
Volume15
Issue number5
Publication statusPublished - 1999 Nov

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Oligodeoxyribonucleotides
Hepatocellular Carcinoma
Messenger RNA
Antigens
Liver
Butyrates
alpha-Fetoproteins
Albumins
Butyric Acid
G1 Phase
Fluorescein
Northern Blotting
Transfection
Cell Cycle
Flow Cytometry
Cell Count
Cell Proliferation
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Antisense oligodeoxynucleotide against c-myc mRNA induces differentiation of human hepatocellular carcinoma cells. / Ebinuma, H.; Saito, Hidetsugu; Saito, Yoshimasa; Wakabayashi, K.; Nakamura, M.; Kurose, I.; Ishii, H.

In: International Journal of Oncology, Vol. 15, No. 5, 11.1999, p. 991-999.

Research output: Contribution to journalArticle

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AU - Ebinuma, H.

AU - Saito, Hidetsugu

AU - Saito, Yoshimasa

AU - Wakabayashi, K.

AU - Nakamura, M.

AU - Kurose, I.

AU - Ishii, H.

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