APC protein is required for initiation of neuronal differentiation in rat pheochromocytoma PC12 cells

Yoh Dobashi, Kazuhiro Katayama, Masataka Kawai, Tetsu Akiyama, Toru Kameya

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The adenomatous polyposis (APC) gene product is highly expressed in the central nervous system. To elucidate the contribution of the APC protein to neuronal differentiation, we used an inducible antisense mRNA vector to suppress APC protein expression and examined neuronal differentiation of PC12 cells induced by nerve growth factor (NGF). When antisense mRNA was induced, APC protein expression was suppressed to 20% of the noninduced level. In those cells, neurite extension induced by NGF and expression of microtubule-associated protein 2 (MAP2) was completely inhibited. However, once cells had differentiated, antisense APC mRNA expression and subsequent suppression of APC protein expression had no effect on either cell morphology or MAP2 protein expression. These results suggest that the wild type APC is critically involved only in the initiation of neuronal differentiation, but not in the maintenance of the differentiated phenotype, or that the neuronal phenotype could be maintained at lower level of APC protein.

Original languageEnglish
Pages (from-to)685-691
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume279
Issue number2
DOIs
Publication statusPublished - 2000 Dec 20

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Keywords

  • APC
  • Antisense
  • Inducible vector
  • Neuronal differentiation
  • PC12 cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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