Apical Cl-/HCO3- exchanger stoichiometry in the modeling of HCO3- transport by pancreatic duct epithelium

Makoto Yamaguchi, Hiroshi Ishiguro, Martin Steward, Yoshiro Sohma, Akiko Yamamoto, Akito Shimouchi, Takaharu Kondo

Research output: Contribution to journalShort survey

6 Citations (Scopus)

Abstract

Pancreatic duct cells secrete a HCO3--rich (∼140 mM) fluid. Using a computer model of the pancreatic duct, Sohma, et al. have demonstrated that the activity of a Cl-/HCO3- exchanger with a 1:1 stoichiometry at the apical membrane would have to be suppressed in order to achieve such a HCO3--rich secretion. Recently the apical exchanger in pancreatic ducts has been identified as SLC26A6 and this probably mediates most of Cl--dependent HCO 3- secretion across the apical membrane. SLC26A6 is reported to mediate electrogenic Cl-/2HCO3- exchange when expressed in Xenopus oocytes. To assess the implications of this 1:2 stoichiometry for HCO3- secretion, we have reconstructed the Sohma model using MATLAB/Simulink. To do this we have formulated an expression for the turnover rate of Cl-/2HCO 3- exchange using network thermodynamics and we have estimated the constants from published experimental data. Preliminary data suggest that the 1:2 stoichiometry of SLC26A6 would favor HCO3 - secretion at higher concentrations.

Original languageEnglish
Pages (from-to)325-328
Number of pages4
JournalJournal of Medical Investigation
Volume56
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2009

Keywords

  • Cl-HCO exchanger
  • Mathematical model
  • Pancreatic duct cell
  • SLC26A6

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Yamaguchi, M., Ishiguro, H., Steward, M., Sohma, Y., Yamamoto, A., Shimouchi, A., & Kondo, T. (2009). Apical Cl-/HCO3- exchanger stoichiometry in the modeling of HCO3- transport by pancreatic duct epithelium. Journal of Medical Investigation, 56(SUPPL. 1), 325-328. https://doi.org/10.2152/jmi.56.325