Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots

Hirohisa Matsushita; Hiroaki Kuwabara; Satoko Ishikawa; Masataka Mochizuki

doi:10.1248/jhs.51.341

Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots

Hirohisa Matsushita, Hiroaki Kuwabara, Satoko Ishikawa, Masataka Mochizuki

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The antiprolife native effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC₅₀ value of 6.6, 5.4,8.7 and 10.2 μg/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.

Original language	English
Pages (from-to)	341-345
Number of pages	5
Journal	Journal of Health Science
Volume	51
Issue number	3
DOIs	https://doi.org/10.1248/jhs.51.341
Publication status	Published - 2005

Keywords

Apoptosis
CCRF-HSB-2
Chlorophytum comosum

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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title = "Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots",

abstract = "The antiprolife native effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC50 value of 6.6, 5.4,8.7 and 10.2 μg/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.",

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AU - Matsushita, Hirohisa

AU - Kuwabara, Hiroaki

AU - Ishikawa, Satoko

AU - Mochizuki, Masataka

PY - 2005

Y1 - 2005

N2 - The antiprolife native effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC50 value of 6.6, 5.4,8.7 and 10.2 μg/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.

AB - The antiprolife native effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC50 value of 6.6, 5.4,8.7 and 10.2 μg/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.

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KW - CCRF-HSB-2

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Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots

Abstract

Keywords

ASJC Scopus subject areas

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