Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots

Hirohisa Matsushita, Hiroaki Kuwabara, Satoko Ishikawa, Masataka Mochizuki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The antiprolife native effects of a n-butanol extract from Chlorophytum comosum (C. comosum) (spider plant) roots was tested in vitro against four human cell lines: HeLa, CCRF-HSB-2, HL-60 and U937 cells. The extract was found to have cell antiproliferative effects against HeLa, CCRF-HSB-2, HL-60 and U937 cell lines, with an IC50 value of 6.6, 5.4,8.7 and 10.2 μg/ml, respectively. The dying cells showed characteristics of apoptosis, such as DNA fragmentation, as assessed by DNA electrophoresis analysis and the Terminal deoxynucleotidyl transferase (TdT)-mediated biotin dUTP Nick End-Labeling (TUNEL) method. These results indicate that extract from C. comosum roots can induce apoptosis in human cell lines.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalJournal of Health Science
Volume51
Issue number3
DOIs
Publication statusPublished - 2005

Fingerprint

Butanols
U937 Cells
HL-60 Cells
Cells
Apoptosis
Cell Line
1-Butanol
Spiders
Plant Roots
DNA Nucleotidylexotransferase
DNA
In Situ Nick-End Labeling
DNA Fragmentation
Biotin
Electrophoresis
Labeling
Inhibitory Concentration 50

Keywords

  • Apoptosis
  • CCRF-HSB-2
  • Chlorophytum comosum

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots. / Matsushita, Hirohisa; Kuwabara, Hiroaki; Ishikawa, Satoko; Mochizuki, Masataka.

In: Journal of Health Science, Vol. 51, No. 3, 2005, p. 341-345.

Research output: Contribution to journalArticle

Matsushita, Hirohisa ; Kuwabara, Hiroaki ; Ishikawa, Satoko ; Mochizuki, Masataka. / Apoptosis induced in human cell lines by a butanol extract from Chlorophytum comosum roots. In: Journal of Health Science. 2005 ; Vol. 51, No. 3. pp. 341-345.
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