Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

Fumihiro Nagashima; Masuo Kondoh; Masaki Kawase; Siro Simizu; Hiroyuki Osada; Makiko Fujii; Yoshiteru Watanabe; Masao Sato; Yoshinori Asakawa

doi:10.1055/s-2003-38888

Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

Fumihiro Nagashima, Masuo Kondoh, Masaki Kawase, Siro Simizu, Hiroyuki Osada, Makiko Fujii, Yoshiteru Watanabe, Masao Sato, Yoshinori Asakawa

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH₂-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

Original language	English
Pages (from-to)	377-379
Number of pages	3
Journal	Planta Medica
Volume	69
Issue number	4
DOIs	https://doi.org/10.1055/s-2003-38888
Publication status	Published - 2003 Apr 1
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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title = "Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata",

abstract = "ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.",

author = "Fumihiro Nagashima and Masuo Kondoh and Masaki Kawase and Siro Simizu and Hiroyuki Osada and Makiko Fujii and Yoshiteru Watanabe and Masao Sato and Yoshinori Asakawa",

year = "2003",

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doi = "10.1055/s-2003-38888",

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AU - Nagashima, Fumihiro

AU - Kondoh, Masuo

AU - Kawase, Masaki

AU - Simizu, Siro

AU - Osada, Hiroyuki

AU - Fujii, Makiko

AU - Watanabe, Yoshiteru

AU - Sato, Masao

AU - Asakawa, Yoshinori

PY - 2003/4/1

Y1 - 2003/4/1

N2 - ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

AB - ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

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ER -

Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

Abstract

ASJC Scopus subject areas

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