Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

Fumihiro Nagashima, Masuo Kondoh, Masaki Kawase, Siro Simizu, Hiroyuki Osada, Makiko Fujii, Yoshiteru Watanabe, Masao Sato, Yoshinori Asakawa

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

Original languageEnglish
Pages (from-to)377-379
Number of pages3
JournalPlanta Medica
Volume69
Issue number4
DOIs
Publication statusPublished - 2003 Apr 1
Externally publishedYes

Fingerprint

Kaurane Diterpenes
Hepatophyta
Diterpenes
diterpenoids
mosses and liverworts
apoptosis
caspases
Apoptosis
HL-60 Cells
Caspases
DNA fragmentation
DNA Fragmentation
cytotoxicity
Chemical activation
Cells
cells
Proteolysis
aspartic proteinases
NAD ADP-ribosyltransferase
Caspase Inhibitors

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata. / Nagashima, Fumihiro; Kondoh, Masuo; Kawase, Masaki; Simizu, Siro; Osada, Hiroyuki; Fujii, Makiko; Watanabe, Yoshiteru; Sato, Masao; Asakawa, Yoshinori.

In: Planta Medica, Vol. 69, No. 4, 01.04.2003, p. 377-379.

Research output: Contribution to journalArticle

Nagashima, F, Kondoh, M, Kawase, M, Simizu, S, Osada, H, Fujii, M, Watanabe, Y, Sato, M & Asakawa, Y 2003, 'Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata', Planta Medica, vol. 69, no. 4, pp. 377-379. https://doi.org/10.1055/s-2003-38888
Nagashima, Fumihiro ; Kondoh, Masuo ; Kawase, Masaki ; Simizu, Siro ; Osada, Hiroyuki ; Fujii, Makiko ; Watanabe, Yoshiteru ; Sato, Masao ; Asakawa, Yoshinori. / Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata. In: Planta Medica. 2003 ; Vol. 69, No. 4. pp. 377-379.
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