Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

Fumihiro Nagashima, Masuo Kondoh, Masaki Kawase, Siro Simizu, Hiroyuki Osada, Makiko Fujii, Yoshiteru Watanabe, Masao Sato, Yoshinori Asakawa

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

Original languageEnglish
Pages (from-to)377-379
Number of pages3
JournalPlanta Medica
Volume69
Issue number4
DOIs
Publication statusPublished - 2003 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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  • Cite this

    Nagashima, F., Kondoh, M., Kawase, M., Simizu, S., Osada, H., Fujii, M., Watanabe, Y., Sato, M., & Asakawa, Y. (2003). Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata. Planta Medica, 69(4), 377-379. https://doi.org/10.1055/s-2003-38888