Abstract
ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 377-379 |
| Number of pages | 3 |
| Journal | Planta Medica |
| Volume | 69 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2003 Apr 1 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Plant Science
- Drug Discovery
- Organic Chemistry
- Pharmacology
Cite this
Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata. / Nagashima, Fumihiro; Kondoh, Masuo; Kawase, Masaki; Simizu, Siro; Osada, Hiroyuki; Fujii, Makiko; Watanabe, Yoshiteru; Sato, Masao; Asakawa, Yoshinori.
In: Planta Medica, Vol. 69, No. 4, 01.04.2003, p. 377-379.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata
AU - Nagashima, Fumihiro
AU - Kondoh, Masuo
AU - Kawase, Masaki
AU - Simizu, Siro
AU - Osada, Hiroyuki
AU - Fujii, Makiko
AU - Watanabe, Yoshiteru
AU - Sato, Masao
AU - Asakawa, Yoshinori
PY - 2003/4/1
Y1 - 2003/4/1
N2 - ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.
AB - ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.
UR - http://www.scopus.com/inward/record.url?scp=0038629444&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038629444&partnerID=8YFLogxK
U2 - 10.1055/s-2003-38888
DO - 10.1055/s-2003-38888
M3 - Article
C2 - 12709910
AN - SCOPUS:0038629444
VL - 69
SP - 377
EP - 379
JO - Planta Medica
JF - Planta Medica
SN - 0032-0943
IS - 4
ER -