Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

Fumihiro Nagashima; Masuo Kondoh; Masaki Kawase; Siro Simizu; Hiroyuki Osada; Makiko Fujii; Yoshiteru Watanabe; Masao Sato; Yoshinori Asakawa

doi:10.1055/s-2003-38888

Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata

Fumihiro Nagashima, Masuo Kondoh, Masaki Kawase, Siro Simizu, Hiroyuki Osada, Makiko Fujii, Yoshiteru Watanabe, Masao Sato, Yoshinori Asakawa

Department of Applied Chemistry

Research output: Contribution to journal › Article

29 Citations (Scopus)

Abstract

ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH₂-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

Original language	English
Pages (from-to)	377-379
Number of pages	3
Journal	Planta Medica
Volume	69
Issue number	4
DOIs	https://doi.org/10.1055/s-2003-38888
Publication status	Published - 2003 Apr 1

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ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

Cite this

Nagashima, F., Kondoh, M., Kawase, M., Simizu, S., Osada, H., Fujii, M., Watanabe, Y., Sato, M., & Asakawa, Y. (2003). Apoptosis-inducing properties of ent-kaurene-type diterpenoids from the liverwort Jungermannia truncata. Planta Medica, 69(4), 377-379. https://doi.org/10.1055/s-2003-38888

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abstract = "ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.",

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AU - Osada, Hiroyuki

AU - Fujii, Makiko

AU - Watanabe, Yoshiteru

AU - Sato, Masao

AU - Asakawa, Yoshinori

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N2 - ent-11α-Hydroxy-16-kauren-15-one (1) induced apoptosis in a human leukemia cell line (HL-60 cells), however, the apoptosis-inducing properties of 1 and its related compounds remain to be proved. We examined the involvement of caspases, a family of cysteine aspartic proteases, which play a central role in induction of apoptosis, in apoptosis induced by the compounds in HL-60 cells. Treatment of the cells with compounds 1, 2 and 3 with the enone group at C-15/C-16 caused DNA fragmentation, a sign of induction of apoptosis, and proteolysis of poly(ADP-ribose) polymerase (PARP), a hallmark of caspase activation. Z-Asp-CH2-DCB, a broad spectrum inhibitor of caspases, abolished the appearance of DNA fragmentation and also significantly attenuated the cytotoxic effects. These data suggest that induction of apoptosis by 1 and some of its related compounds are dependent on caspases activation and might be partly involved in the cytotoxicity in HL-60 cells.

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10.1055/s-2003-38888