Apoptosis of human embryonal carcinoma cells with in vitro differentiation

Taketo Yamada, Nao Suzuki, Nobuyoshi Hiraoka, Kentaroh Matsuoka, Sachiko Fukushima, Akinori Hashiguchi, Jun Ichi Hata

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An in vitro model of apoptosis and differentiation in human embryonal carcinoma (EC) cells was developed to study the mode of cell death and mechanisms of cell death in early development. Death of these cells was induced by treatment with retinoic acid (RA) under the same conditions as those for induction of differentiation. The manner of this cell death was apoptosis, not necrosis, with the morphological criterion for apoptosis. Serum deprivation likewise caused apoptosis in both undifferentiated and differentiated EC cells. In differentiated EC cells, DNA fragmentation was observed in a smear pattern lacking the ladder pattern typically associated with apoptosis. However, in differentiated EC cells, DNA fragmentation occurred in various sizes. The expression of a carbohydrate antigen, Le(Y), a reported marker of apoptotic cancer cells, was increased by the treatment with RA. However, two-color analysis by Bow cytometry with nick end labelling method revealed that Le(Y) expression was closely correlated with cellular differentiation but not apoptosis after RA treatment in the human EC cell system. Collection of Le(Y) positive cells by the magnetic bead method demonstrated that this expression was not due to apoptosis but rather to differentiation. On the other hand, Le(Y) expression associated with apoptosis was induced by serum deprivation in both undifferentiated and differentiated EC cells. These results show that a subpopulation of undifferentiated EC cells takes the apoptotic pathway by induction of differentiation. The results also suggest that the population of cells taking an apoptotic pathway differs from a population of cells taking a differentiation pathway. This in vitro system for apoptosis in human EC cells will be useful for studies concerning apoptosis or programmed cell death in human developmental biology.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalCell Structure and Function
Volume21
Issue number1
Publication statusPublished - 1996 Feb

Fingerprint

Embryonal Carcinoma Stem Cells
Apoptosis
Cell Death
Tretinoin
DNA Fragmentation
In Vitro Techniques
Developmental Biology
Serum
Population
Necrosis
Color
Carbohydrates
Carcinoma

Keywords

  • Apoptosis
  • Cellular differentiation
  • Human embryonal carcinoma cell
  • Le(Y)
  • Retinoic acid

ASJC Scopus subject areas

  • Physiology
  • Structural Biology
  • Cell Biology

Cite this

Yamada, T., Suzuki, N., Hiraoka, N., Matsuoka, K., Fukushima, S., Hashiguchi, A., & Hata, J. I. (1996). Apoptosis of human embryonal carcinoma cells with in vitro differentiation. Cell Structure and Function, 21(1), 53-61.

Apoptosis of human embryonal carcinoma cells with in vitro differentiation. / Yamada, Taketo; Suzuki, Nao; Hiraoka, Nobuyoshi; Matsuoka, Kentaroh; Fukushima, Sachiko; Hashiguchi, Akinori; Hata, Jun Ichi.

In: Cell Structure and Function, Vol. 21, No. 1, 02.1996, p. 53-61.

Research output: Contribution to journalArticle

Yamada, T, Suzuki, N, Hiraoka, N, Matsuoka, K, Fukushima, S, Hashiguchi, A & Hata, JI 1996, 'Apoptosis of human embryonal carcinoma cells with in vitro differentiation', Cell Structure and Function, vol. 21, no. 1, pp. 53-61.
Yamada T, Suzuki N, Hiraoka N, Matsuoka K, Fukushima S, Hashiguchi A et al. Apoptosis of human embryonal carcinoma cells with in vitro differentiation. Cell Structure and Function. 1996 Feb;21(1):53-61.
Yamada, Taketo ; Suzuki, Nao ; Hiraoka, Nobuyoshi ; Matsuoka, Kentaroh ; Fukushima, Sachiko ; Hashiguchi, Akinori ; Hata, Jun Ichi. / Apoptosis of human embryonal carcinoma cells with in vitro differentiation. In: Cell Structure and Function. 1996 ; Vol. 21, No. 1. pp. 53-61.
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