Appearance of reverse transcriptase activity in human fetal thymus cells stimulated by human b-cell

Akira Ono, Hidetsugu Saito, Masaharu Tsuchiya, Tatsuya Nakamura, Toru Higashinakagawa, Shunzo Kondo, Hiroshi Yoshimatsu

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Abstract

We have investigated three human fetal thymuses by various induction procedures in a search for retrovirus. Thymus cells were cultured for 7 days together with mitomycin C-treated B cells and examined for reverse transcriptase activity. The following results were obtained. 1) RNA-directed DNA polymerase (RDDP) activity was detected in the culture medium on day 3 and persisted until day 7. This enzyme, after partial purification on phos-phocellulose column, showed template-primer specificity of a typical reverse transcriptase. 2) In unstimulated thymus cells, no reverse transcriptase activity was detected. 3) Stimulation with lymphocyte mitogens such as phytohaemagglutinin (PHA) and concanavalin A (Con A) exerted no effect on inducing the reverse transcriptase activity. 4) No reverse transcriptase activity was obtained in the culture medium of B cells pretreated with mitomycin C. Taken together these results indicate that in human fetal thymus cells reverse transcriptase activity is induced by human B cell stimulation, pointing to an intriguing possibility of protovirus activation.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalThe Keio journal of medicine
Volume32
DOIs
Publication statusPublished - 1983

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RNA-Directed DNA Polymerase
Human Activities
Thymus Gland
B-Lymphocytes
Mitomycin
Culture Media
Phytohemagglutinins
Retroviridae
Lymphocyte Activation
Concanavalin A
Mitogens
Cultured Cells
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

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Appearance of reverse transcriptase activity in human fetal thymus cells stimulated by human b-cell. / Ono, Akira; Saito, Hidetsugu; Tsuchiya, Masaharu; Nakamura, Tatsuya; Higashinakagawa, Toru; Kondo, Shunzo; Yoshimatsu, Hiroshi.

In: The Keio journal of medicine, Vol. 32, 1983, p. 73-80.

Research output: Contribution to journalArticle

Ono, Akira ; Saito, Hidetsugu ; Tsuchiya, Masaharu ; Nakamura, Tatsuya ; Higashinakagawa, Toru ; Kondo, Shunzo ; Yoshimatsu, Hiroshi. / Appearance of reverse transcriptase activity in human fetal thymus cells stimulated by human b-cell. In: The Keio journal of medicine. 1983 ; Vol. 32. pp. 73-80.
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AU - Tsuchiya, Masaharu

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AU - Higashinakagawa, Toru

AU - Kondo, Shunzo

AU - Yoshimatsu, Hiroshi

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