Application of cationic latex particles for protein separation

Yoshiteru Sumi, Toshifumi Shiroya, Keiji Fujimoto, Tadashi Wada, Hiroshi Handa, Haruma Kawaguchi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have prepared cationic latex particles with amino groups on their surface. The particles were composed of a polystyrene core and a poly(glycidyl methacrylate) surface layer, to which hexamethylenediamine was immobilized by means of coupling with epoxy groups. The zeta potential of the particles was approximately 30 mV and the isoelectric point was 10.5. The latex particles preferentially adsorbed acidic proteins through an electrostatic interaction. We compared the capacity and selectivity in adsorption of acidic proteins on the cationic particles and on diethylaminoethyl (DEAE)-Sepharose gel, which has been conventionally used. The latex particles were twenty times more efficient in adsorption of proteins than DEAE-Sepharose gel. Acidic proteins encoded by the adenovirus Ela gene were selectively separated from crude cell extracts using the cationic latex particles.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume2
Issue number4
DOIs
Publication statusPublished - 1994 May 9

Keywords

  • Cationic latex particles
  • Protein separation

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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