Application of chemical cytochrome P450 model systems to studies on drug metabolism .VI: N, N-coupling reaction of N-methylaniline catalyzed by polypeptide-bound porphyrinatoiron(III) and cytochrome P450

Takuko Doi, Takashi Mori, Tadamko Mashino, Masaaki Hirobe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Polypeptide-bound porphyrinatoiron(III) effectively catalyzed the N, N coupling reaction of N-methylaniline in the presence of NaBH4/O2/tetramethyl-ammonium hydroxide or cumenehydroperoxide, whereas the corresponding non-bound monomer porphyrinatoiron(III) had little catalytic activity. Rat liver microsomes also catalyzed the N, N-coupling which was inhibited by cytochrome P-450 specific inhibitors, SKF-525A and metyrapone. The polypeptide-bound porphinatoiron(III) appears to be an excellent cytochrome P450 model for drug metabolism studies.

Original languageEnglish
Pages (from-to)737-743
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number2
DOIs
Publication statusPublished - 1993 Mar 15
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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