Application of Chemical Cytochrome P450 Model Systems to Studies on Drug Metabolism .VI: N,N-Coupling Reaction of N-Methylaniline Catalyzed by Polypeptide-Bound Porphyrinatoiron(III) and Cytochrome P450

T. Doi, T. Mori, Tadahiko Mashino, M. Hirobe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Polypeptide-bound porphyrinatoiron(III) effectively catalyzed the N,N coupling reaction of N-methylaniline in the presence of NaBH4/O2/tetramethyl-ammonium hydroxide or cumenehydroperoxide, whereas the corresponding non-bound monomer porphyrinatoiron(III) had little catalytic activity. Rat liver microsomes also catalyzed the N,N-coupling which was inhibited by cytochrome P-450 specific inhibitors, SKF-525A and metyrapone. The polypeptide-bound porphinatoiron(III) appears to be an excellent cytochrome P450 model for drug metabolism studies.

Original languageEnglish
Pages (from-to)737-743
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number2
DOIs
Publication statusPublished - 1993 Mar 15
Externally publishedYes

Fingerprint

Metabolism
Cytochrome P-450 Enzyme System
Ammonium Hydroxide
Proadifen
Metyrapone
Peptides
Liver Microsomes
Pharmaceutical Preparations
Liver
Rats
Catalyst activity
Monomers
methylaniline
Cytochrome P-450 Enzyme Inhibitors

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

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T2 - N,N-Coupling Reaction of N-Methylaniline Catalyzed by Polypeptide-Bound Porphyrinatoiron(III) and Cytochrome P450

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AU - Mori, T.

AU - Mashino, Tadahiko

AU - Hirobe, M.

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