Application of mesenchymal stem cells for the regeneration of cardiomyocyte and its use for cell transplantation therapy.

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Abstract

We have isolated a cardiomyogenic cell line (CMG cell) from murine bone marrow mesenchymal stem cells. The cells showed a fibroblast-like morphology, but the morphology changed after 5-azacytidine exposure. They began spontaneous beating after 2 weeks, and expressed ANP and BNP. Electron microscopy revealed a cardiomyocyte-like ultrastructure. These cells had several types of action potentials; sinus node-like and ventricular cell-like action potentials. The isoform of contractile protein genes indicated that their muscle phenotype was similar to fetal ventricular cardiomyocytes. They expressed alpha1A, alpha1B, alpha1D, beta1, and beta2 adrenergic and M1 and M2 muscarinic receptors. Stimulation with phenylephrine, isoproterenol and carbachol increased ERK phosphorylation and second messengers. Isoproterenol increased the beating rate, which was blocked with CGP20712A (beta1-selective blocker). These findings indicated that cell transplantation therapy for the patients with heart failure might possibly be achieved using the regenerated cardiomyocytes from autologous bone marrow cells in the near future.

Original languageEnglish
Pages (from-to)83-94
Number of pages12
JournalHuman cell : official journal of Human Cell Research Society
Volume16
Issue number3
Publication statusPublished - 2003 Sep

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Cell Transplantation
Cell- and Tissue-Based Therapy
Mesenchymal Stromal Cells
Cardiac Myocytes
Regeneration
Isoproterenol
Action Potentials
Muscarinic M2 Receptors
Muscarinic M1 Receptors
Azacitidine
Contractile Proteins
Sinoatrial Node
Carbachol
Atrial Natriuretic Factor
Second Messenger Systems
Phenylephrine
Bone Marrow Cells
Adrenergic Agents
Electron Microscopy
Protein Isoforms

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

Cite this

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abstract = "We have isolated a cardiomyogenic cell line (CMG cell) from murine bone marrow mesenchymal stem cells. The cells showed a fibroblast-like morphology, but the morphology changed after 5-azacytidine exposure. They began spontaneous beating after 2 weeks, and expressed ANP and BNP. Electron microscopy revealed a cardiomyocyte-like ultrastructure. These cells had several types of action potentials; sinus node-like and ventricular cell-like action potentials. The isoform of contractile protein genes indicated that their muscle phenotype was similar to fetal ventricular cardiomyocytes. They expressed alpha1A, alpha1B, alpha1D, beta1, and beta2 adrenergic and M1 and M2 muscarinic receptors. Stimulation with phenylephrine, isoproterenol and carbachol increased ERK phosphorylation and second messengers. Isoproterenol increased the beating rate, which was blocked with CGP20712A (beta1-selective blocker). These findings indicated that cell transplantation therapy for the patients with heart failure might possibly be achieved using the regenerated cardiomyocytes from autologous bone marrow cells in the near future.",
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