Application of monoclonal antibodies for endothelin to hypertensive research

Yoshihiko Saito, Kazuwa Nakao, Masashi Mukoyama, Gotaro Shirakami, Hiroshi Itoh, Takayuki Yamada, Hiroshi Arai, Kiminori Hosoda, Shin Ichi Suga, Michihisa Jougasaki, Yoshihiro Ogawa, Shigeyuki Nakajima, Motohiko Ueda, Hiroo Imura

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

We developed six kinds of monoclonal antibodies against endothelin (ET)-1 recognizing different epitopes with high affinities (5×1010 M-1 to 5×1011 M-1). Using these monoclonal antibodies, we developed radioimmunoassays for ET-1 with different specificities. Cross-reactivities with ET-2 ranged from 80% to 100%, and those with ET-3 ranged from 3% to 60% Patients with essential hypertension (n=20) showed a significant elevation in the plasma ET-1-LI level compared with age-matched control subjects (n=12) (30.1±1.4 pg/ml versus 18.5±0.9 pg/ml, p<0.01). The Plasma ET-1-LI level in hypertensive patients in stages II and III (World Health Organization classification) was significantly higher than that in those patients in stage I. There was no significant correlation between the plasma ET-1-LI level and systolic blood pressure (r=0.11), diastolic blood pressure (r=-0.13), or age (r=0.24) in all patients studied who had essential hypertension. In the neutralization experiment, monoclonal antibodies attenuated ET-1-induced contraction of rat aortic rings and the pressor action of ET-1 in pithed rats in vivo. The present study demonstrates the elevated plasma ET-1-LI level in patients with essential hypertension. Monoclonal antibodies developed in this study can become powerful tools to investigate the pathophysiological significance of ET in essential hypertension.

Original languageEnglish
Pages (from-to)734-738
Number of pages5
JournalHypertension
Volume15
Issue number6 PART 2
Publication statusPublished - 1990 Jun
Externally publishedYes

Fingerprint

Endothelins
Endothelin-1
Monoclonal Antibodies
Research
Blood Pressure
Endothelin-2
Endothelin-3
Radioimmunoassay
Epitopes
Essential Hypertension

Keywords

  • Endothelin
  • Essential hypertension
  • Monoclonal antibodies
  • Neutralization tests

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Saito, Y., Nakao, K., Mukoyama, M., Shirakami, G., Itoh, H., Yamada, T., ... Imura, H. (1990). Application of monoclonal antibodies for endothelin to hypertensive research. Hypertension, 15(6 PART 2), 734-738.

Application of monoclonal antibodies for endothelin to hypertensive research. / Saito, Yoshihiko; Nakao, Kazuwa; Mukoyama, Masashi; Shirakami, Gotaro; Itoh, Hiroshi; Yamada, Takayuki; Arai, Hiroshi; Hosoda, Kiminori; Suga, Shin Ichi; Jougasaki, Michihisa; Ogawa, Yoshihiro; Nakajima, Shigeyuki; Ueda, Motohiko; Imura, Hiroo.

In: Hypertension, Vol. 15, No. 6 PART 2, 06.1990, p. 734-738.

Research output: Contribution to journalArticle

Saito, Y, Nakao, K, Mukoyama, M, Shirakami, G, Itoh, H, Yamada, T, Arai, H, Hosoda, K, Suga, SI, Jougasaki, M, Ogawa, Y, Nakajima, S, Ueda, M & Imura, H 1990, 'Application of monoclonal antibodies for endothelin to hypertensive research', Hypertension, vol. 15, no. 6 PART 2, pp. 734-738.
Saito Y, Nakao K, Mukoyama M, Shirakami G, Itoh H, Yamada T et al. Application of monoclonal antibodies for endothelin to hypertensive research. Hypertension. 1990 Jun;15(6 PART 2):734-738.
Saito, Yoshihiko ; Nakao, Kazuwa ; Mukoyama, Masashi ; Shirakami, Gotaro ; Itoh, Hiroshi ; Yamada, Takayuki ; Arai, Hiroshi ; Hosoda, Kiminori ; Suga, Shin Ichi ; Jougasaki, Michihisa ; Ogawa, Yoshihiro ; Nakajima, Shigeyuki ; Ueda, Motohiko ; Imura, Hiroo. / Application of monoclonal antibodies for endothelin to hypertensive research. In: Hypertension. 1990 ; Vol. 15, No. 6 PART 2. pp. 734-738.
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abstract = "We developed six kinds of monoclonal antibodies against endothelin (ET)-1 recognizing different epitopes with high affinities (5×1010 M-1 to 5×1011 M-1). Using these monoclonal antibodies, we developed radioimmunoassays for ET-1 with different specificities. Cross-reactivities with ET-2 ranged from 80{\%} to 100{\%}, and those with ET-3 ranged from 3{\%} to 60{\%} Patients with essential hypertension (n=20) showed a significant elevation in the plasma ET-1-LI level compared with age-matched control subjects (n=12) (30.1±1.4 pg/ml versus 18.5±0.9 pg/ml, p<0.01). The Plasma ET-1-LI level in hypertensive patients in stages II and III (World Health Organization classification) was significantly higher than that in those patients in stage I. There was no significant correlation between the plasma ET-1-LI level and systolic blood pressure (r=0.11), diastolic blood pressure (r=-0.13), or age (r=0.24) in all patients studied who had essential hypertension. In the neutralization experiment, monoclonal antibodies attenuated ET-1-induced contraction of rat aortic rings and the pressor action of ET-1 in pithed rats in vivo. The present study demonstrates the elevated plasma ET-1-LI level in patients with essential hypertension. Monoclonal antibodies developed in this study can become powerful tools to investigate the pathophysiological significance of ET in essential hypertension.",
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