Application of quantitative real-time PCR for monitoring the process of enrichment of clones on in vitro protein selection

Kenichi Horisawa, Nobuhide Doi, Hideaki Takashima, Hiroshi Yanagawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In vitro selection of proteins from cDNA libraries using display technologies, such as the in vitro virus method, is a powerful means for the discovery of novel protein interactions. After iterative screening, selected proteins are usually identified and evaluated by cloning and sequencing analysis. Previously we applied real-time PCR for evaluation of the sequences obtained on in vitro virus screening. Here, we have presented additional data regarding monitoring of the process of enrichment of selected clones in each round of selection and elimination of false positives by real-time PCR, and have also discussed the utility of the novel method. This approach should also be applicable to other display technologies.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalJournal of Biochemistry
Volume137
Issue number2
DOIs
Publication statusPublished - 2005 Feb

Fingerprint

Real-Time Polymerase Chain Reaction
Clone Cells
Viruses
Monitoring
Screening
Display devices
Technology
Proteins
Cloning
Gene Library
Organism Cloning
In Vitro Techniques

Keywords

  • Display technology
  • False positive
  • in vitro virus
  • mRNA display
  • Real-time PCR

ASJC Scopus subject areas

  • Biochemistry

Cite this

Application of quantitative real-time PCR for monitoring the process of enrichment of clones on in vitro protein selection. / Horisawa, Kenichi; Doi, Nobuhide; Takashima, Hideaki; Yanagawa, Hiroshi.

In: Journal of Biochemistry, Vol. 137, No. 2, 02.2005, p. 121-124.

Research output: Contribution to journalArticle

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