Application of recombination transfer to the cognate Bacillus subtilis genome.

Satoshi Tomita; Kenji Tsuge; Y. Kikuchi; Mitsuhiro Itaya

doi:10.1093/nass/3.1.295

Application of recombination transfer to the cognate Bacillus subtilis genome.

Satoshi Tomita, Kenji Tsuge, Y. Kikuchi, Mitsuhiro Itaya

Research output: Contribution to journal › Article › peer-review

Abstract

Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are cloned in the BReT vector. The BReT plasmid recovered the predicted genome sequence as large as 100 kb with high fidelity. The result indicates that the BReT system originally developed to recover the non-cognate segments cloned in the B. subtilis genome vector can be applied to the cognate sequence.

Original language	English
Pages (from-to)	295-296
Number of pages	2
Journal	Nucleic acids research. Supplement (2001)
Issue number	3
DOIs	https://doi.org/10.1093/nass/3.1.295
Publication status	Published - 2003
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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abstract = "Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are cloned in the BReT vector. The BReT plasmid recovered the predicted genome sequence as large as 100 kb with high fidelity. The result indicates that the BReT system originally developed to recover the non-cognate segments cloned in the B. subtilis genome vector can be applied to the cognate sequence.",

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AU - Tsuge, Kenji

AU - Kikuchi, Y.

AU - Itaya, Mitsuhiro

PY - 2003

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AB - Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are cloned in the BReT vector. The BReT plasmid recovered the predicted genome sequence as large as 100 kb with high fidelity. The result indicates that the BReT system originally developed to recover the non-cognate segments cloned in the B. subtilis genome vector can be applied to the cognate sequence.

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JO - Nucleic acids research. Supplement (2001)

JF - Nucleic acids research. Supplement (2001)

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ER -

Application of recombination transfer to the cognate Bacillus subtilis genome.

Abstract

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