Application to the preparation of useful compounds based on enzyme- And microorganism-mediated reactions

Ken Ichi Fuhshuku, Shigeru Nishiyama, Takeshi Sugai

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4 Citations (Scopus)


This article describes our recent studies on enzyme- and microorganism-mediated reactions, and application to the preparation of useful compounds based on the complementary chemo-enzymatic synthesis. First, lipase-catalyzed reactions were focused especially on the preparation of enantiomerically pure compounds, such as N-Boc-indoline-2-carboxylic acid, indanoxazolidinone and symbioramide. Next, the control of nitrile-hydrolyzing enzyme in Rhodococcus rhodochrous was summarized. A one-pot chemo-enzymatic transformation of aldehydes to amides was established. The third topic is the application of yeast-mediated asymmetric reduction of carbonyl compounds. The preparation of a couple of enantiomerically pure starting materials for natural product synthesis by the combination with chemical transformation was disclosed.

Original languageEnglish
Pages (from-to)664-676
Number of pages13
JournalYuki Gosei Kagaku Kyokaishi/Journal of Synthetic Organic Chemistry
Issue number6
Publication statusPublished - 2006 Jun



  • Amidase
  • Amide formation
  • Asymmetric reduction
  • Chemo-enzymatic synthesis
  • Hydrolysis
  • Kinetic resolution
  • Lipase
  • Nitrile hydratase
  • Yeast

ASJC Scopus subject areas

  • Organic Chemistry

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