Abstract
A novel and simple method that combines an online concentration technique with an enantioseparation technique for capillary electrophoresis-namely, cation-selective exhaustive injection and sweeping cyclodextrin-modified micellar electrokinetic chromatography (CSEI-sweeping CD-modified MEKC)-realizes the effective enantioseparation of cationic analytes while keeping a significant increase of detection sensitivity. This technique consists of a slight modification of the basic CSEI-sweeping MEKC. The main idea is to simply add an anionic CD as a chiral selector into the micellar buffer including sodium dodecyl sulfate, but not to change any other buffers in order to preserve the online concentration mechanism. When applied to analysis of the street drug, methamphetamine, the method achieved not only a baseline enantioseparation but also limits of detection (LODs; S/N = 3) of 70-90 pg/mL (ppt) for each isomer. This translates to a more than 10 000-fold improvement compared to the LODs by the usual injection method. The present technique, which was made from a slight modification of CSEI-sweeping MEKC, would give an attractive approach that is applicable to almost any analytes for which CSEI-sweeping MEKC is applicable; all that is required is the selection of an appropriate anionic CD to be added to the micellar buffer.
| Original language | English |
|---|---|
| Journal | Electrophoresis |
| DOIs | |
| Publication status | Accepted/In press - 2016 |
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Keywords
- Cation-selective exhaustive injection
- CD-modified micellar electrokinetic chromatography
- Chiral analysis
- Online concentration
- Sweeping
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry
Cite this
Approaching over 10 000-fold sensitivity increase in chiral capillary electrophoresis : Cation-selective exhaustive injection and sweeping cyclodextrin-modified micellar electrokinetic chromatography. / Mikuma, Toshiyasu; Iwata, Yuko T.; Miyaguchi, Hajime; Kuwayama, Kenji; Tsujikawa, Kenji; Kanamori, Tatsuyuki; Kanazawa, Hideko; Inoue, Hiroyuki.
In: Electrophoresis, 2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Approaching over 10 000-fold sensitivity increase in chiral capillary electrophoresis
T2 - Cation-selective exhaustive injection and sweeping cyclodextrin-modified micellar electrokinetic chromatography
AU - Mikuma, Toshiyasu
AU - Iwata, Yuko T.
AU - Miyaguchi, Hajime
AU - Kuwayama, Kenji
AU - Tsujikawa, Kenji
AU - Kanamori, Tatsuyuki
AU - Kanazawa, Hideko
AU - Inoue, Hiroyuki
PY - 2016
Y1 - 2016
N2 - A novel and simple method that combines an online concentration technique with an enantioseparation technique for capillary electrophoresis-namely, cation-selective exhaustive injection and sweeping cyclodextrin-modified micellar electrokinetic chromatography (CSEI-sweeping CD-modified MEKC)-realizes the effective enantioseparation of cationic analytes while keeping a significant increase of detection sensitivity. This technique consists of a slight modification of the basic CSEI-sweeping MEKC. The main idea is to simply add an anionic CD as a chiral selector into the micellar buffer including sodium dodecyl sulfate, but not to change any other buffers in order to preserve the online concentration mechanism. When applied to analysis of the street drug, methamphetamine, the method achieved not only a baseline enantioseparation but also limits of detection (LODs; S/N = 3) of 70-90 pg/mL (ppt) for each isomer. This translates to a more than 10 000-fold improvement compared to the LODs by the usual injection method. The present technique, which was made from a slight modification of CSEI-sweeping MEKC, would give an attractive approach that is applicable to almost any analytes for which CSEI-sweeping MEKC is applicable; all that is required is the selection of an appropriate anionic CD to be added to the micellar buffer.
AB - A novel and simple method that combines an online concentration technique with an enantioseparation technique for capillary electrophoresis-namely, cation-selective exhaustive injection and sweeping cyclodextrin-modified micellar electrokinetic chromatography (CSEI-sweeping CD-modified MEKC)-realizes the effective enantioseparation of cationic analytes while keeping a significant increase of detection sensitivity. This technique consists of a slight modification of the basic CSEI-sweeping MEKC. The main idea is to simply add an anionic CD as a chiral selector into the micellar buffer including sodium dodecyl sulfate, but not to change any other buffers in order to preserve the online concentration mechanism. When applied to analysis of the street drug, methamphetamine, the method achieved not only a baseline enantioseparation but also limits of detection (LODs; S/N = 3) of 70-90 pg/mL (ppt) for each isomer. This translates to a more than 10 000-fold improvement compared to the LODs by the usual injection method. The present technique, which was made from a slight modification of CSEI-sweeping MEKC, would give an attractive approach that is applicable to almost any analytes for which CSEI-sweeping MEKC is applicable; all that is required is the selection of an appropriate anionic CD to be added to the micellar buffer.
KW - Cation-selective exhaustive injection
KW - CD-modified micellar electrokinetic chromatography
KW - Chiral analysis
KW - Online concentration
KW - Sweeping
UR - http://www.scopus.com/inward/record.url?scp=84986268057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986268057&partnerID=8YFLogxK
U2 - 10.1002/elps.201600324
DO - 10.1002/elps.201600324
M3 - Article
C2 - 27542560
AN - SCOPUS:84986268057
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
ER -