Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells

Sachiko Watanabe, Catharina Sagita Moniaga, Søren Nielsen, Mariko Chikuma

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Aquaporin (AQP) 9, a member of the transmembrane water channel family, is defined as a water/glycerol transporting protein. Some AQPs including AQP3 and AQP8 have been recently found to transport hydrogen peroxide (H2O2). Here we show that AQP9 facilitates the membrane transport of H2O2 in human and mice cells. Enforced expression of human AQP9 in Chinese hamster ovary-K1 potentiated the increase in cellular H2O2 after adding exogenous H2O2. In contrast, AQP9 knockdown by siRNA in human hepatoma HepG2 cells reduced the import of extracellular H2O2. In addition, the uptake of extracellular H2O2 was suppressed in erythrocytes and bone marrow-derived mast cells from AQP9 knockout mice compared with wild-type cells. Coincidentally, H2O2-induced cytotoxicity was attenuated by AQP9 deficiency in human and mice cells. Our findings implicate the involvement of AQP9 in H2O2 transport in human and mice cells.

Original languageEnglish
Pages (from-to)191-197
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume471
Issue number1
DOIs
Publication statusPublished - 2016 Feb 26
Externally publishedYes

Fingerprint

Aquaporins
Hydrogen Peroxide
Cells
Membranes
Cytotoxicity
Glycerol
Small Interfering RNA
Bone
Water
Hep G2 Cells
Cricetulus
Proteins
Knockout Mice
Mast Cells
Hepatocellular Carcinoma
Ovary
Erythrocytes
Bone Marrow

Keywords

  • Aquaporin 9
  • Hydrogen peroxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells. / Watanabe, Sachiko; Moniaga, Catharina Sagita; Nielsen, Søren; Chikuma, Mariko.

In: Biochemical and Biophysical Research Communications, Vol. 471, No. 1, 26.02.2016, p. 191-197.

Research output: Contribution to journalArticle

Watanabe, Sachiko ; Moniaga, Catharina Sagita ; Nielsen, Søren ; Chikuma, Mariko. / Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 471, No. 1. pp. 191-197.
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