Arginase II Expressed in Cancer-Associated Fibroblasts Indicates Tissue Hypoxia and Predicts Poor Outcome in Patients with Pancreatic Cancer

Yoshinori Ino, Rie Yamazaki-Itoh, Seiji Oguro, Kazuaki Shimada, Tomoo Kosuge, Jan Zavada, Yae Kanai, Nobuyoshi Hiraoka

Research output: Contribution to journalArticle

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Abstract

An adequate level of arginine in the tissue microenvironment is essential for T cell activity and survival. Arginine levels are regulated by the arginine-catabolizing enzyme, arginase (ARG). It has been reported that arginase II (ARG2), one of two ARGs, is aberrantly expressed in prostate cancer cells, which convert arginine into ornithine, resulting in a lack of arginine that weakens tumor-infiltrating lymphocytes and renders them dysfunctional. However, immune suppression mediated by ARG2-expressing cancer cells in lung cancer has not been observed. Here we studied the expression of ARG2 in pancreatic ductal carcinoma (PDC) tissue clinicopathologically by examining over 200 cases of PDC. In contrast to prostate cancer, ARG2 expression was rarely demonstrated in PDC cells by immunohistochemistry, and instead ARG2 was characteristically expressed in α-smooth muscle actin-positive cancer-associated fibroblasts (CAFs), especially those located within and around necrotic areas in PDC. The presence of ARG2-expressing CAFs was closely correlated with shorter overall survival (OS; P = 0.003) and disease-free survival (DFS; P = 0.0006). Multivariate Cox regression analysis showed that the presence of ARG2-expressing CAFs in PDC tissue was an independent predictor of poorer OS (hazard ratio [HR] = 1.582, P = 0.007) and DFS (HR = 1.715, P = 0.001) in PDC patients. In addition to the characteristic distribution of ARG2-expressing CAFs, such CAFs co-expressed carbonic anhydrase IX, SLC2A1, or HIF-1α, markers of hypoxia, in PDC tissue. Furthermore, in vitro experiments revealed that cultured fibroblasts extracted from PDC tissue expressed the ARG2 transcript after exposure to hypoxia, which had arginase activity. These results indicate that cancer cell-mediated immune suppression through ARG2 expression is not a general event and that the presence of ARG2-expressing CAFs is an indicator of poor prognosis, as well as hypoxia, in PDC tissue.

Original languageEnglish
Article numbere55146
JournalPLoS One
Volume8
Issue number2
DOIs
Publication statusPublished - 2013 Feb 12
Externally publishedYes

Fingerprint

Pancreatic Ductal Carcinoma
Arginase
arginase
pancreatic neoplasms
Fibroblasts
Pancreatic Neoplasms
carcinoma
fibroblasts
hypoxia
Tissue
Arginine
neoplasms
arginine
Cells
prostatic neoplasms
Hazards
Prostatic Neoplasms
Ornithine
Carbonic Anhydrases
T-cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Arginase II Expressed in Cancer-Associated Fibroblasts Indicates Tissue Hypoxia and Predicts Poor Outcome in Patients with Pancreatic Cancer. / Ino, Yoshinori; Yamazaki-Itoh, Rie; Oguro, Seiji; Shimada, Kazuaki; Kosuge, Tomoo; Zavada, Jan; Kanai, Yae; Hiraoka, Nobuyoshi.

In: PLoS One, Vol. 8, No. 2, e55146, 12.02.2013.

Research output: Contribution to journalArticle

Ino, Yoshinori ; Yamazaki-Itoh, Rie ; Oguro, Seiji ; Shimada, Kazuaki ; Kosuge, Tomoo ; Zavada, Jan ; Kanai, Yae ; Hiraoka, Nobuyoshi. / Arginase II Expressed in Cancer-Associated Fibroblasts Indicates Tissue Hypoxia and Predicts Poor Outcome in Patients with Pancreatic Cancer. In: PLoS One. 2013 ; Vol. 8, No. 2.
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AU - Oguro, Seiji

AU - Shimada, Kazuaki

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AU - Kanai, Yae

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