Arginine remarkably prolongs the lifetime of the M-intermediate in the bacteriorhodopsin photocycle at room temperature

Masayoshi Nakasako, Mikio Kataoka, Fumio Tokunaga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The lifetime of the M-intermediate in the bacteriorhodopsin photocycle was remarkably prolonged on drying a suspension of purple membrane, in arginine solution at alkaline pH. The M-intermediate could be completely accumulated under illumination (530 nm) at room temperature. The crystalline structure of purple membranes was retained after this treatment. The lifetime of the M-intermediate was found to be longer than 100 s and depended on the pH of the purple membrane suspension before drying. It was suggested that an interaction between the guanidium group of arginine and an amino acid residue played an important role in the prolongation.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalFEBS Letters
Volume254
Issue number1-2
DOIs
Publication statusPublished - 1989 Aug 28
Externally publishedYes

Fingerprint

Purple Membrane
Bacteriorhodopsins
Arginine
Membranes
Temperature
Suspensions
Drying
Lighting
Crystalline materials
Amino Acids

Keywords

  • Arginine
  • Bacteriorhodopsin
  • M-intermediate
  • Purple membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Arginine remarkably prolongs the lifetime of the M-intermediate in the bacteriorhodopsin photocycle at room temperature. / Nakasako, Masayoshi; Kataoka, Mikio; Tokunaga, Fumio.

In: FEBS Letters, Vol. 254, No. 1-2, 28.08.1989, p. 211-214.

Research output: Contribution to journalArticle

@article{abd88808fe9b4b06b3d02de79dd44c57,
title = "Arginine remarkably prolongs the lifetime of the M-intermediate in the bacteriorhodopsin photocycle at room temperature",
abstract = "The lifetime of the M-intermediate in the bacteriorhodopsin photocycle was remarkably prolonged on drying a suspension of purple membrane, in arginine solution at alkaline pH. The M-intermediate could be completely accumulated under illumination (530 nm) at room temperature. The crystalline structure of purple membranes was retained after this treatment. The lifetime of the M-intermediate was found to be longer than 100 s and depended on the pH of the purple membrane suspension before drying. It was suggested that an interaction between the guanidium group of arginine and an amino acid residue played an important role in the prolongation.",
keywords = "Arginine, Bacteriorhodopsin, M-intermediate, Purple membrane",
author = "Masayoshi Nakasako and Mikio Kataoka and Fumio Tokunaga",
year = "1989",
month = "8",
day = "28",
doi = "10.1016/0014-5793(89)81041-5",
language = "English",
volume = "254",
pages = "211--214",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Arginine remarkably prolongs the lifetime of the M-intermediate in the bacteriorhodopsin photocycle at room temperature

AU - Nakasako, Masayoshi

AU - Kataoka, Mikio

AU - Tokunaga, Fumio

PY - 1989/8/28

Y1 - 1989/8/28

N2 - The lifetime of the M-intermediate in the bacteriorhodopsin photocycle was remarkably prolonged on drying a suspension of purple membrane, in arginine solution at alkaline pH. The M-intermediate could be completely accumulated under illumination (530 nm) at room temperature. The crystalline structure of purple membranes was retained after this treatment. The lifetime of the M-intermediate was found to be longer than 100 s and depended on the pH of the purple membrane suspension before drying. It was suggested that an interaction between the guanidium group of arginine and an amino acid residue played an important role in the prolongation.

AB - The lifetime of the M-intermediate in the bacteriorhodopsin photocycle was remarkably prolonged on drying a suspension of purple membrane, in arginine solution at alkaline pH. The M-intermediate could be completely accumulated under illumination (530 nm) at room temperature. The crystalline structure of purple membranes was retained after this treatment. The lifetime of the M-intermediate was found to be longer than 100 s and depended on the pH of the purple membrane suspension before drying. It was suggested that an interaction between the guanidium group of arginine and an amino acid residue played an important role in the prolongation.

KW - Arginine

KW - Bacteriorhodopsin

KW - M-intermediate

KW - Purple membrane

UR - http://www.scopus.com/inward/record.url?scp=0001188245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001188245&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(89)81041-5

DO - 10.1016/0014-5793(89)81041-5

M3 - Article

AN - SCOPUS:0001188245

VL - 254

SP - 211

EP - 214

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-2

ER -