Arginine remarkably prolongs the lifetime of the M-intermediate in the bacteriorhodopsin photocycle at room temperature

Masayoshi Nakasako, Mikio Kataoka, Fumio Tokunaga

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The lifetime of the M-intermediate in the bacteriorhodopsin photocycle was remarkably prolonged on drying a suspension of purple membrane, in arginine solution at alkaline pH. The M-intermediate could be completely accumulated under illumination (530 nm) at room temperature. The crystalline structure of purple membranes was retained after this treatment. The lifetime of the M-intermediate was found to be longer than 100 s and depended on the pH of the purple membrane suspension before drying. It was suggested that an interaction between the guanidium group of arginine and an amino acid residue played an important role in the prolongation.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalFEBS Letters
Volume254
Issue number1-2
DOIs
Publication statusPublished - 1989 Aug 28
Externally publishedYes

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Keywords

  • Arginine
  • Bacteriorhodopsin
  • M-intermediate
  • Purple membrane

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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