Arginine vasopressin inhibits fluid secretion in guinea pig pancreatic duct cells

Shigeru Ko, S. Naruse, M. Kitagawa, H. Ishiguro, M. Murakami, T. Hayakawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effects of arginine vasopressin (AVP) on pancreatic ductal secretion were studied in guinea pigs. In the isolated vascularly perfused pancreas, AVP reduced secretin-stimulated fluid secretion and increased the vascular resistance when the perfusion rate was held constant. In the isolated interlobular duct segments, AVP inhibited secretin-stimulated fluid secretion, indicating the direct inhibitory action of AVP on the duct cells. AVP affected neither the basal nor the secretin-induced cAMP productions, suggesting that AVP inhibits the fluid secretion at a point distal to the production of cAMP. AVP increased intracellular Ca2+ concentration ([Ca2+](i)) in the absence of extracellular Ca2+. When [Ca2+](i) was elevated by the application of thapsigargin, AVP caused a rapid decrease in [Ca2+](i). AVP seems to activate both Ca2+ release from intracellular stores and Ca2+ efflux across the plasma membrane, but its relation to the inhibition of fluid secretion remains to be clarified. It is concluded that AVP directly inhibits secretin-stimulated ductal fluid secretion in the guinea pig pancreas.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume277
Issue number1 40-1
Publication statusPublished - 1999 Jul
Externally publishedYes

Fingerprint

Fluids and Secretions
Arginine Vasopressin
Pancreatic Ducts
Guinea Pigs
Secretin
Pancreas
Thapsigargin
Vascular Resistance
Perfusion
Cell Membrane

Keywords

  • Adenosine 3',5'-cyclic monophosphate
  • Intracellular calcium concentration
  • Isolated interlobular duct segment
  • Isolated vascularly perfused pancreas
  • Luminal volume

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

Arginine vasopressin inhibits fluid secretion in guinea pig pancreatic duct cells. / Ko, Shigeru; Naruse, S.; Kitagawa, M.; Ishiguro, H.; Murakami, M.; Hayakawa, T.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 277, No. 1 40-1, 07.1999.

Research output: Contribution to journalArticle

Ko, Shigeru ; Naruse, S. ; Kitagawa, M. ; Ishiguro, H. ; Murakami, M. ; Hayakawa, T. / Arginine vasopressin inhibits fluid secretion in guinea pig pancreatic duct cells. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1999 ; Vol. 277, No. 1 40-1.
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