Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm of mouse embryos

Miho Oka, Keisuke Hashimoto, Yoshifumi Yamaguchi, Shin Ichiro Saitoh, Yuki Sugiura, Yuji Motoi, Kurara Honda, Yorifumi Kikko, Shinya Ohata, Makoto Suematsu, Masayuki Miura, Kensuke Miyake, Toshiaki Katada, Kenji Kontani

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The small GTPase Arl8b localizes primarily to lysosomes and is involved in lysosomal motility and fusion. Here, we show that Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm (VYSE), an apical cell layer of the visceral yolk sac, of mouse embryos. The VYSE actively takes up maternal materials from uterine fluid and degrades them in lysosomes to provide breakdown products to the embryo. Arl8b gene-trap mice (Arl8b-/-) displayed decreased early embryo body size. The Arl8b-/- VYSE exhibited defective endocytic trafficking to the lysosome and accumulation of maternal proteins such as albumin and immunoglobulin G in late endocytic organelles. Furthermore, Transthyretin-Cre;Arl8bflox/flox mice in which Arl8b was ablated specifically in the VYSE also showed decreased embryo body size, defects in trafficking to the lysosome and reduction of the free amino acid level in the embryos. Taken together, these results suggest that Arl8b mediates lysosomal degradation of maternal proteins in the VYSE, thereby contributing to mouse embryonic development.

Original languageEnglish
Pages (from-to)3568-3577
Number of pages10
JournalJournal of Cell Science
Volume130
Issue number20
DOIs
Publication statusPublished - 2017

Fingerprint

Yolk Sac
Endoderm
Proteolysis
Embryonic Structures
Mothers
Lysosomes
Body Size
Prealbumin
Monomeric GTP-Binding Proteins
Organelles
Embryonic Development
Albumins
Immunoglobulin G
Amino Acids
Genes
Proteins

Keywords

  • Embryogenesis
  • Endocytosis
  • Lysosome
  • Metabolomics
  • Small GTPase

ASJC Scopus subject areas

  • Cell Biology

Cite this

Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm of mouse embryos. / Oka, Miho; Hashimoto, Keisuke; Yamaguchi, Yoshifumi; Saitoh, Shin Ichiro; Sugiura, Yuki; Motoi, Yuji; Honda, Kurara; Kikko, Yorifumi; Ohata, Shinya; Suematsu, Makoto; Miura, Masayuki; Miyake, Kensuke; Katada, Toshiaki; Kontani, Kenji.

In: Journal of Cell Science, Vol. 130, No. 20, 2017, p. 3568-3577.

Research output: Contribution to journalArticle

Oka, M, Hashimoto, K, Yamaguchi, Y, Saitoh, SI, Sugiura, Y, Motoi, Y, Honda, K, Kikko, Y, Ohata, S, Suematsu, M, Miura, M, Miyake, K, Katada, T & Kontani, K 2017, 'Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm of mouse embryos', Journal of Cell Science, vol. 130, no. 20, pp. 3568-3577. https://doi.org/10.1242/jcs.200519
Oka, Miho ; Hashimoto, Keisuke ; Yamaguchi, Yoshifumi ; Saitoh, Shin Ichiro ; Sugiura, Yuki ; Motoi, Yuji ; Honda, Kurara ; Kikko, Yorifumi ; Ohata, Shinya ; Suematsu, Makoto ; Miura, Masayuki ; Miyake, Kensuke ; Katada, Toshiaki ; Kontani, Kenji. / Arl8b is required for lysosomal degradation of maternal proteins in the visceral yolk sac endoderm of mouse embryos. In: Journal of Cell Science. 2017 ; Vol. 130, No. 20. pp. 3568-3577.
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