Abstract
Piwi and piRNA form the piRNA-induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–piRISC precursor (pre-piRISC) to mitochondria, where Zucchini-dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre-piRISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre-piRISC from Yb bodies and the translocation to mitochondria. Piwi–pre-piRISC resist leaving Yb bodies until Armi binds Piwi–pre-piRISC through the piRNA precursors. The lack of the Armi N-terminus also blocks the Piwi–pre-piRISC exit from Yb bodies. Thus, Armi determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter-organelle translocation for maturation.
| Original language | English |
|---|---|
| Article number | e48769 |
| Journal | EMBO Reports |
| DOIs | |
| Publication status | Accepted/In press - 2019 Jan 1 |
Fingerprint
Keywords
- Armitage
- Gasz
- mitochondrion
- piRNA
- Yb body
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics
Cite this
Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter-organelle translocation. / Yamashiro, Haruna; Negishi, Mayu; Kinoshita, Tatsuki; Ishizu, Hirotsugu; Ohtani, Hitoshi; Siomi, Mikiko C.
In: EMBO Reports, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter-organelle translocation
AU - Yamashiro, Haruna
AU - Negishi, Mayu
AU - Kinoshita, Tatsuki
AU - Ishizu, Hirotsugu
AU - Ohtani, Hitoshi
AU - Siomi, Mikiko C.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Piwi and piRNA form the piRNA-induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–piRISC precursor (pre-piRISC) to mitochondria, where Zucchini-dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre-piRISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre-piRISC from Yb bodies and the translocation to mitochondria. Piwi–pre-piRISC resist leaving Yb bodies until Armi binds Piwi–pre-piRISC through the piRNA precursors. The lack of the Armi N-terminus also blocks the Piwi–pre-piRISC exit from Yb bodies. Thus, Armi determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter-organelle translocation for maturation.
AB - Piwi and piRNA form the piRNA-induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–piRISC precursor (pre-piRISC) to mitochondria, where Zucchini-dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre-piRISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre-piRISC from Yb bodies and the translocation to mitochondria. Piwi–pre-piRISC resist leaving Yb bodies until Armi binds Piwi–pre-piRISC through the piRNA precursors. The lack of the Armi N-terminus also blocks the Piwi–pre-piRISC exit from Yb bodies. Thus, Armi determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter-organelle translocation for maturation.
KW - Armitage
KW - Gasz
KW - mitochondrion
KW - piRNA
KW - Yb body
UR - http://www.scopus.com/inward/record.url?scp=85076336299&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076336299&partnerID=8YFLogxK
U2 - 10.15252/embr.201948769
DO - 10.15252/embr.201948769
M3 - Article
AN - SCOPUS:85076336299
JO - EMBO Reports
JF - EMBO Reports
SN - 1469-221X
M1 - e48769
ER -