Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter-organelle translocation

Haruna Yamashiro, Mayu Negishi, Tatsuki Kinoshita, Hirotsugu Ishizu, Hitoshi Ohtani, Mikiko C. Siomi

Research output: Contribution to journalArticle

Abstract

Piwi and piRNA form the piRNA-induced silencing complex (piRISC) to repress transposons. In the current model, Armitage (Armi) brings the Piwi–piRISC precursor (pre-piRISC) to mitochondria, where Zucchini-dependent piRISC maturation occurs. Here, we show that Armi is necessary for Piwi–pre-piRISC formation at Yb bodies and that Armi triggers the exit of Piwi–pre-piRISC from Yb bodies and the translocation to mitochondria. Piwi–pre-piRISC resist leaving Yb bodies until Armi binds Piwi–pre-piRISC through the piRNA precursors. The lack of the Armi N-terminus also blocks the Piwi–pre-piRISC exit from Yb bodies. Thus, Armi determines Piwi–piRISC processing, in a multilayered manner, from precursor formation and quality control to inter-organelle translocation for maturation.

Original languageEnglish
Article numbere48769
JournalEMBO Reports
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Quality Control
Organelles
Small Interfering RNA
Quality control
Processing
Mitochondria

Keywords

  • Armitage
  • Gasz
  • mitochondrion
  • piRNA
  • Yb body

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter-organelle translocation. / Yamashiro, Haruna; Negishi, Mayu; Kinoshita, Tatsuki; Ishizu, Hirotsugu; Ohtani, Hitoshi; Siomi, Mikiko C.

In: EMBO Reports, 01.01.2019.

Research output: Contribution to journalArticle

Yamashiro, Haruna ; Negishi, Mayu ; Kinoshita, Tatsuki ; Ishizu, Hirotsugu ; Ohtani, Hitoshi ; Siomi, Mikiko C. / Armitage determines Piwi−piRISC processing from precursor formation and quality control to inter-organelle translocation. In: EMBO Reports. 2019.
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AU - Ohtani, Hitoshi

AU - Siomi, Mikiko C.

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