Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lung

Wei Xia Peng, Tatsuhiro Shibata, Hiroto Katoh, Akiko Kokubu, Yoshihiro Matsuno, Hisao Asamura, Ryosuke Tsuchiya, Yae Kanai, Fumie Hosoda, Tokuki Sakiyama, Misao Ohki, Issei Imoto, Johji Inazawa, Setsuo Hirohashi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We examined a large number of primary high-grade neuroendocrine tumors of the lung (10 small cell lung carcinomas and 31 large cell neuroendocrine carcinomas) by using array-based comparative genomic hybridization using microarrays spotted with 800 bacterial artificial chromosome clones containing tumor-related genes from throughout the human genome. We identified the genome-wide copy number alteration profiles of these tumors, including recurrent amplifications located at 2q21.2, 3q21-27, 3q26, 3q27-29, 5p14.2, 5p13, 7q21.1, 8q21, and 8q24 and homozygous deletions at 1p36, 4p16, 4p16.3, 9p21.3, 9p21, 19p13.3, and 20q13. Our results revealed that small cell lung carcinomas and large cell neuroendocrine carcinomas have multiple characteristic chromosomal alterations in common, but that distinctive alterations also exist between the two subtypes. Moreover, we found that the two subtypes undergo different processes of accumulating these genetic alterations during tumor development. By comparing the genetic profiles with the clinicopathological features, we discovered many chromosomal loci whose alterations were significantly associated with clinical stage and patient prognosis. These results will be valuable for evaluating clinical status, including patient prognosis, and for identifying novel molecular targets for effective therapies.

Original languageEnglish
Pages (from-to)661-667
Number of pages7
JournalCancer Science
Volume96
Issue number10
DOIs
Publication statusPublished - 2005 Oct
Externally publishedYes

Fingerprint

Comparative Genomic Hybridization
Neuroendocrine Tumors
Neuroendocrine Carcinoma
Large Cell Carcinoma
Small Cell Lung Carcinoma
Lung
Genetic Phenomena
Bacterial Artificial Chromosomes
Neoplasms
Human Genome
Clone Cells
Genome
Genes
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lung. / Peng, Wei Xia; Shibata, Tatsuhiro; Katoh, Hiroto; Kokubu, Akiko; Matsuno, Yoshihiro; Asamura, Hisao; Tsuchiya, Ryosuke; Kanai, Yae; Hosoda, Fumie; Sakiyama, Tokuki; Ohki, Misao; Imoto, Issei; Inazawa, Johji; Hirohashi, Setsuo.

In: Cancer Science, Vol. 96, No. 10, 10.2005, p. 661-667.

Research output: Contribution to journalArticle

Peng, WX, Shibata, T, Katoh, H, Kokubu, A, Matsuno, Y, Asamura, H, Tsuchiya, R, Kanai, Y, Hosoda, F, Sakiyama, T, Ohki, M, Imoto, I, Inazawa, J & Hirohashi, S 2005, 'Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lung', Cancer Science, vol. 96, no. 10, pp. 661-667. https://doi.org/10.1111/j.1349-7006.2005.00092.x
Peng, Wei Xia ; Shibata, Tatsuhiro ; Katoh, Hiroto ; Kokubu, Akiko ; Matsuno, Yoshihiro ; Asamura, Hisao ; Tsuchiya, Ryosuke ; Kanai, Yae ; Hosoda, Fumie ; Sakiyama, Tokuki ; Ohki, Misao ; Imoto, Issei ; Inazawa, Johji ; Hirohashi, Setsuo. / Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lung. In: Cancer Science. 2005 ; Vol. 96, No. 10. pp. 661-667.
@article{cc4cccd60e0d4262b2043a1cd8b6d1b9,
title = "Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lung",
abstract = "We examined a large number of primary high-grade neuroendocrine tumors of the lung (10 small cell lung carcinomas and 31 large cell neuroendocrine carcinomas) by using array-based comparative genomic hybridization using microarrays spotted with 800 bacterial artificial chromosome clones containing tumor-related genes from throughout the human genome. We identified the genome-wide copy number alteration profiles of these tumors, including recurrent amplifications located at 2q21.2, 3q21-27, 3q26, 3q27-29, 5p14.2, 5p13, 7q21.1, 8q21, and 8q24 and homozygous deletions at 1p36, 4p16, 4p16.3, 9p21.3, 9p21, 19p13.3, and 20q13. Our results revealed that small cell lung carcinomas and large cell neuroendocrine carcinomas have multiple characteristic chromosomal alterations in common, but that distinctive alterations also exist between the two subtypes. Moreover, we found that the two subtypes undergo different processes of accumulating these genetic alterations during tumor development. By comparing the genetic profiles with the clinicopathological features, we discovered many chromosomal loci whose alterations were significantly associated with clinical stage and patient prognosis. These results will be valuable for evaluating clinical status, including patient prognosis, and for identifying novel molecular targets for effective therapies.",
author = "Peng, {Wei Xia} and Tatsuhiro Shibata and Hiroto Katoh and Akiko Kokubu and Yoshihiro Matsuno and Hisao Asamura and Ryosuke Tsuchiya and Yae Kanai and Fumie Hosoda and Tokuki Sakiyama and Misao Ohki and Issei Imoto and Johji Inazawa and Setsuo Hirohashi",
year = "2005",
month = "10",
doi = "10.1111/j.1349-7006.2005.00092.x",
language = "English",
volume = "96",
pages = "661--667",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "10",

}

TY - JOUR

T1 - Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lung

AU - Peng, Wei Xia

AU - Shibata, Tatsuhiro

AU - Katoh, Hiroto

AU - Kokubu, Akiko

AU - Matsuno, Yoshihiro

AU - Asamura, Hisao

AU - Tsuchiya, Ryosuke

AU - Kanai, Yae

AU - Hosoda, Fumie

AU - Sakiyama, Tokuki

AU - Ohki, Misao

AU - Imoto, Issei

AU - Inazawa, Johji

AU - Hirohashi, Setsuo

PY - 2005/10

Y1 - 2005/10

N2 - We examined a large number of primary high-grade neuroendocrine tumors of the lung (10 small cell lung carcinomas and 31 large cell neuroendocrine carcinomas) by using array-based comparative genomic hybridization using microarrays spotted with 800 bacterial artificial chromosome clones containing tumor-related genes from throughout the human genome. We identified the genome-wide copy number alteration profiles of these tumors, including recurrent amplifications located at 2q21.2, 3q21-27, 3q26, 3q27-29, 5p14.2, 5p13, 7q21.1, 8q21, and 8q24 and homozygous deletions at 1p36, 4p16, 4p16.3, 9p21.3, 9p21, 19p13.3, and 20q13. Our results revealed that small cell lung carcinomas and large cell neuroendocrine carcinomas have multiple characteristic chromosomal alterations in common, but that distinctive alterations also exist between the two subtypes. Moreover, we found that the two subtypes undergo different processes of accumulating these genetic alterations during tumor development. By comparing the genetic profiles with the clinicopathological features, we discovered many chromosomal loci whose alterations were significantly associated with clinical stage and patient prognosis. These results will be valuable for evaluating clinical status, including patient prognosis, and for identifying novel molecular targets for effective therapies.

AB - We examined a large number of primary high-grade neuroendocrine tumors of the lung (10 small cell lung carcinomas and 31 large cell neuroendocrine carcinomas) by using array-based comparative genomic hybridization using microarrays spotted with 800 bacterial artificial chromosome clones containing tumor-related genes from throughout the human genome. We identified the genome-wide copy number alteration profiles of these tumors, including recurrent amplifications located at 2q21.2, 3q21-27, 3q26, 3q27-29, 5p14.2, 5p13, 7q21.1, 8q21, and 8q24 and homozygous deletions at 1p36, 4p16, 4p16.3, 9p21.3, 9p21, 19p13.3, and 20q13. Our results revealed that small cell lung carcinomas and large cell neuroendocrine carcinomas have multiple characteristic chromosomal alterations in common, but that distinctive alterations also exist between the two subtypes. Moreover, we found that the two subtypes undergo different processes of accumulating these genetic alterations during tumor development. By comparing the genetic profiles with the clinicopathological features, we discovered many chromosomal loci whose alterations were significantly associated with clinical stage and patient prognosis. These results will be valuable for evaluating clinical status, including patient prognosis, and for identifying novel molecular targets for effective therapies.

UR - http://www.scopus.com/inward/record.url?scp=28444472003&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28444472003&partnerID=8YFLogxK

U2 - 10.1111/j.1349-7006.2005.00092.x

DO - 10.1111/j.1349-7006.2005.00092.x

M3 - Article

VL - 96

SP - 661

EP - 667

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 10

ER -