Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

Takuya Terai, Moe Kohno, Gaelle Boncompain, Shigeru Sugiyama, Nae Saito, Ryo Fujikake, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Takayoshi Okabe, Yasuteru Urano, Franck Perez, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Artificial ligands of streptavidin (ALiS) with association constants of 106 M-1 were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.

Original languageEnglish
Pages (from-to)10464-10467
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number33
DOIs
Publication statusPublished - 2015 Aug 11
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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