Aryl hydrocarbon receptor negatively regulates dendritic cell immunogenicity via a kynurenine-dependent mechanism

Nam Trung Nguyen, Akihiro Kimura, Taisuke Nakahama, Ichino Chinen, Kazuya Masuda, Keiko Nohara, Yoshiaki Fujii-Kuriyama, Tadamitsu Kishimoto

Research output: Contribution to journalArticle

327 Citations (Scopus)

Abstract

Although an immunoregulatory role of aryl hydrocarbon receptor (Ahr) has been demonstrated in T cells and macrophages, little is known about its function in dendritic cells (DC). Here, we show that lipopolysaccharide (LPS) and CpG stimulate Ahr expression in bone marrow-derived dendritic cells (BMDC). Furthermore, we found that Ahr is required to induce indoleamine 2,3-dioxygenase (IDO) expression, an immunosuppressive enzyme that catabolizes tryptophan into kynurenine (Kyn) and other metabolites in DC. In the presence of LPS or CpG, Ahr-deficient (Ahr-/-) mature BMDC induced immune responses characterized by reduced Kyn and IL-10 production compared with results observed with tolerogenic mature WT BMDC. In a coculture system with LPS- or CpG-stimulated BMDC and naive T cells, Ahr-/- BMDC inhibited naive T-cell differentiation into regulatory T (Treg) cells, which likely facilitated Th17 cell development and promoted naive T-cell proliferation. Addition of synthetic L-Kyn to the coculture system skewed the differentiation of naive T cells to Treg cells rather than Th17 cells. Taken together, our results demonstrate a previously unknown negatively regulatory role for Ahr in DC-mediated immunogenesis in the presence of LPS or CpG, which, in turn, alters the Kyn-dependent generation of Treg cells and Th17 cells from naive T cells.

Original languageEnglish
Pages (from-to)19961-19966
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number46
DOIs
Publication statusPublished - 2010 Nov 16

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Kynurenine
Aryl Hydrocarbon Receptors
Dendritic Cells
Regulatory T-Lymphocytes
T-Lymphocytes
Th17 Cells
Bone Marrow
Lipopolysaccharides
Coculture Techniques
Indoleamine-Pyrrole 2,3,-Dioxygenase
Immunosuppressive Agents
Tryptophan
Interleukin-10
Cell Differentiation
Macrophages
Cell Proliferation

Keywords

  • Dioxin receptor
  • Immune regulation
  • Tryptophan catabolism

ASJC Scopus subject areas

  • General

Cite this

Aryl hydrocarbon receptor negatively regulates dendritic cell immunogenicity via a kynurenine-dependent mechanism. / Nguyen, Nam Trung; Kimura, Akihiro; Nakahama, Taisuke; Chinen, Ichino; Masuda, Kazuya; Nohara, Keiko; Fujii-Kuriyama, Yoshiaki; Kishimoto, Tadamitsu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 46, 16.11.2010, p. 19961-19966.

Research output: Contribution to journalArticle

Nguyen, Nam Trung ; Kimura, Akihiro ; Nakahama, Taisuke ; Chinen, Ichino ; Masuda, Kazuya ; Nohara, Keiko ; Fujii-Kuriyama, Yoshiaki ; Kishimoto, Tadamitsu. / Aryl hydrocarbon receptor negatively regulates dendritic cell immunogenicity via a kynurenine-dependent mechanism. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 46. pp. 19961-19966.
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