Ascorbate free radical reductase activity in vertebrate lenses of some species

Shuji Matsukura, Masayasu Bando, Hajime Obazawa, Mikako Oka, Makoto Takehana

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose : To clarify the function of ascorbate free radical (AFR) reductase in the lens antioxidation mechanism, we investigated the difference among species in AFR reductase activity in different vertebrate lenses. Materials and Methods : Soluble and insoluble fractions were prepared from the lenses of frogs, guinea pigs, rats, rabbits, pigs, and calves. AFR reductase and diaphorase activity of each fraction was determined. Results : AFR reductase activity in the lens soluble fraction was the highest in frogs. That of guinea pigs and rabbits was at the next level ; there was only a little activity in rats and pigs, and none was detected in calves. Membrane-bound AFR reductase in the lens insoluble fraction was extracted by 0.3% Triton X - 100. The membrane - bound enzyme activity was almost at the same level in frogs, rats, rabbits, and calves, and a little higher in guinea pigs and pigs. However, such species-specificity of AFR reductase activity as in the soluble fraction was not observed in 0.3% Triton X - 100 extracts. Diaphorasc activity was 3 to 9 times as much as AFR reductasc activity in the soluble fractions of frogs, guinea pigs, and rabbits, but in 0.3% Triton X - 100 extracts of all vertebrate species used, it was very high, 108 to 311 times the AFR reductase activity. Conclusion : These results suggest that the lens soluble and membrane-bound AFR reductases are individual enzyme molecules and have different antioxidative functions. The lenses of frogs, guinea pigs, and rabbits contain a near-ultraviolet (UV) light absorbing compound, reduced pyridine nucleotide at a high concentration. Therefore, the soluble AFR reductase activity may be high in the vertebrate lenses with a near-UV light filter, and enhance the antinho-toxidation of ascorbic acid.

Original languageEnglish
Pages (from-to)384-389
Number of pages6
JournalJournal of Japanese Ophthalmological Society
Volume104
Issue number6
Publication statusPublished - 2000 Dec 1

Keywords

  • Antioxidation
  • Ascorbate free radical reductase
  • Diaphorase
  • Lens
  • Near-UV light filter

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Matsukura, S., Bando, M., Obazawa, H., Oka, M., & Takehana, M. (2000). Ascorbate free radical reductase activity in vertebrate lenses of some species. Journal of Japanese Ophthalmological Society, 104(6), 384-389.