Ascorbate stimulates monooxygenase-dependent steroidogenesis in adrenal zona glomerulosa

Fumiko Mitani, Tadashi Ogishima, Kuniaki Mukai, Makoto Suematsu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It is well known that ascorbic acid (Asc) is highly concentrated in the adrenal gland, but its function in the gland is not thoroughly elucidated. We therefore examined the possibility that Asc participates in steroidogenic monooxygenase systems of the adrenal cortex with the aid of the regenerating system including outer mitochondrial membrane cytochrome b (OMb). When Asc availability was limited in rat mutants unable to synthesize Asc, the increase in plasma aldosterone concentration under Na-deficiency was suppressed without effect on plasma corticosterone concentration. Aldosterone formation in the isolated mitochondrial fraction of the zona glomerulosa (zG) of the adrenal cortex was stimulated by the addition of Asc and NADH, while corticosterone formation was not. Consistently zG showed a high level of Asc regeneration activity and was rich in OMb among adrenocortical zones. Taken together, the enhanced aldosterone formation that is catalyzed by one of the steroidogenic monooxygenases, P450aldo, may be supported by Asc with its regenerating system.

Original languageEnglish
Pages (from-to)483-490
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number1
DOIs
Publication statusPublished - 2005 Dec 9

Fingerprint

Zona Glomerulosa
Mixed Function Oxygenases
Ascorbic Acid
Aldosterone
Adrenal Cortex
Corticosterone
Plasmas
Cytochromes b
Mitochondrial Membranes
Adrenal Glands
NAD
Rats
Regeneration
Availability
Membranes

Keywords

  • Adrenal cortex
  • Aldosterone formation
  • Aldosterone synthase cytochrome P450aldo
  • Ascorbic acid
  • Outer mitochondrial membrane cytochrome b
  • Semidehydroascorbic acid reductase
  • Steroidogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ascorbate stimulates monooxygenase-dependent steroidogenesis in adrenal zona glomerulosa. / Mitani, Fumiko; Ogishima, Tadashi; Mukai, Kuniaki; Suematsu, Makoto.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 1, 09.12.2005, p. 483-490.

Research output: Contribution to journalArticle

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