Aspergillus species strain M39 produces two naphtho-γ-pyrones that reverse drug resistance in human KB cells

S. I. Ikeda, M. Sugita, Akihiko Yoshimura, T. Sumizawa, H. Douzono, Y. Nagata, S. I. Akiyama

Research output: Contribution to journalArticle

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Abstract

One thousand fungi and Actinomycetes were investigated to see whether they produced compounds that reverse multidrug resistance in KB cells. Only one Aspergillus strain M39 produced agents with resistance-reversing activity and these compounds were identified to be rubrofusarin B and dianhydro-aurasperone C. Rubrofusarin B only slightly reversed the resistance of KB-C2 cells to Adriamycin and daunomycin, partially reversed the resistance to chromomycin A3, and almost completely reversed the resistance to vincristine and mitomycin C. Purified dianhydro-aurasperone C and rubrofusarin B had similar effects on drug resistance in KB-8-5 cells. Dianhydro-aurasperone C enhanced the accumulation of vinblastine in KB-8-5 cells and inhibited the efflux of vinblastine from the cells. Dianhydro-aurasperone C and rubrofusarin B at 10 μm completely inhibited 3H-azidopine photolabelling of P-glycoprotein. The two products of Aspergillus strain M39 appear to reverse multi-drug resistance by interacting with P-glycoprotein and inhibiting its role as an active efflux pump.

Original languageEnglish
Pages (from-to)508-513
Number of pages6
JournalInternational Journal of Cancer
Volume45
Issue number3
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Pyrones
KB Cells
Aspergillus
Drug Resistance
Vinblastine
Multiple Drug Resistance
P-Glycoprotein
Chromomycin A3
Daunorubicin
Actinobacteria
Mitomycin
Vincristine
Doxorubicin
Fungi
rubrofusarin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ikeda, S. I., Sugita, M., Yoshimura, A., Sumizawa, T., Douzono, H., Nagata, Y., & Akiyama, S. I. (1990). Aspergillus species strain M39 produces two naphtho-γ-pyrones that reverse drug resistance in human KB cells. International Journal of Cancer, 45(3), 508-513.

Aspergillus species strain M39 produces two naphtho-γ-pyrones that reverse drug resistance in human KB cells. / Ikeda, S. I.; Sugita, M.; Yoshimura, Akihiko; Sumizawa, T.; Douzono, H.; Nagata, Y.; Akiyama, S. I.

In: International Journal of Cancer, Vol. 45, No. 3, 1990, p. 508-513.

Research output: Contribution to journalArticle

Ikeda, SI, Sugita, M, Yoshimura, A, Sumizawa, T, Douzono, H, Nagata, Y & Akiyama, SI 1990, 'Aspergillus species strain M39 produces two naphtho-γ-pyrones that reverse drug resistance in human KB cells', International Journal of Cancer, vol. 45, no. 3, pp. 508-513.
Ikeda, S. I. ; Sugita, M. ; Yoshimura, Akihiko ; Sumizawa, T. ; Douzono, H. ; Nagata, Y. ; Akiyama, S. I. / Aspergillus species strain M39 produces two naphtho-γ-pyrones that reverse drug resistance in human KB cells. In: International Journal of Cancer. 1990 ; Vol. 45, No. 3. pp. 508-513.
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