Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography

Akira Kono, Setsuro Sugata, Yasuhiro Hara, Mutsuko Tanaka, Yoshiharu Karube, Yoshikazu Matsushima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5’-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0. and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.

Original languageEnglish
Pages (from-to)1666-1669
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume31
Issue number5
DOIs
Publication statusPublished - 1983 Jan 1

Keywords

  • 5-chlorouracil
  • 5-fluorouracil
  • 5-fluorouridine
  • 5’-deoxy-5-fluorouridine
  • HPLC
  • thymidine
  • thymidine phosphorylase
  • thymine
  • uracil
  • uridine
  • uridine phosphorylase

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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