Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography

Akira Kono; Setsuro Sugata; Yasuhiro Hara; Mutsuko Tanaka; Yoshiharu Karube; Yoshikazu Matsushima

doi:10.1248/cpb.31.1666

Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography

Akira Kono, Setsuro Sugata, Yasuhiro Hara, Mutsuko Tanaka, Yoshiharu Karube, Yoshikazu Matsushima

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5’-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0. and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.

Original language	English
Pages (from-to)	1666-1669
Number of pages	4
Journal	Chemical and Pharmaceutical Bulletin
Volume	31
Issue number	5
DOIs	https://doi.org/10.1248/cpb.31.1666
Publication status	Published - 1983 Jan 1

Keywords

5-chlorouracil
5-fluorouracil
5-fluorouridine
5’-deoxy-5-fluorouridine
HPLC
thymidine
thymidine phosphorylase
thymine
uracil
uridine
uridine phosphorylase

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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10.1248/cpb.31.1666

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title = "Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography",

abstract = "A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5{\textquoteright}-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0. and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.",

keywords = "5-chlorouracil, 5-fluorouracil, 5-fluorouridine, 5{\textquoteright}-deoxy-5-fluorouridine, HPLC, thymidine, thymidine phosphorylase, thymine, uracil, uridine, uridine phosphorylase",

author = "Akira Kono and Setsuro Sugata and Yasuhiro Hara and Mutsuko Tanaka and Yoshiharu Karube and Yoshikazu Matsushima",

year = "1983",

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T1 - Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography

AU - Kono, Akira

AU - Sugata, Setsuro

AU - Hara, Yasuhiro

AU - Tanaka, Mutsuko

AU - Karube, Yoshiharu

AU - Matsushima, Yoshikazu

PY - 1983/1/1

Y1 - 1983/1/1

N2 - A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5’-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0. and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.

AB - A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5’-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0. and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.

KW - 5-chlorouracil

KW - 5-fluorouracil

KW - 5-fluorouridine

KW - 5’-deoxy-5-fluorouridine

KW - HPLC

KW - thymidine

KW - thymidine phosphorylase

KW - thymine

KW - uracil

KW - uridine

KW - uridine phosphorylase

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DO - 10.1248/cpb.31.1666

M3 - Article

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SN - 0009-2363

VL - 31

SP - 1666

EP - 1669

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

IS - 5

ER -

Assay for Pyrimidine Nucleoside Phosphorylases in Tissue Extracts by High-Performance Liquid Chromatography

Abstract

Keywords

ASJC Scopus subject areas

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