Assays of protonemal growth responses in physcomitrella patens under blue- and red-light stimuli

Sho Miyazaki, Masatoshi Nakajima, Hiroshi Kawaide

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The non-seed land plant Physcomitrella patens is a model species for developmental, cellular, and molecular biology studies in mosses and also for performing genetic analyses. Previously, it was shown that wild-type P. patens displays a unique photomorphogenetic behavior, in which chloronemal filaments grow in the opposite direction to a blue-light source. Here, we describe bioassay systems that can be used to study light avoidance responses as well as other aspects of photomorphogenetic regulation in P. patens grown under red- and blue-light sources.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-43
Number of pages9
DOIs
Publication statusPublished - 2019 Jan 1
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1924
ISSN (Print)1064-3745

Fingerprint

Bryopsida
Light
Growth
Embryophyta
Bryophyta
Developmental Biology
Biological Assay
Cell Biology
Molecular Biology

Keywords

  • Blue light
  • Bryophyte
  • Caulonema
  • Chloronema
  • Gibberellin
  • Moss
  • Photomorphogenesis
  • Physcomitrella
  • Protonema
  • Red light

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Miyazaki, S., Nakajima, M., & Kawaide, H. (2019). Assays of protonemal growth responses in physcomitrella patens under blue- and red-light stimuli. In Methods in Molecular Biology (pp. 35-43). (Methods in Molecular Biology; Vol. 1924). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9015-3_4

Assays of protonemal growth responses in physcomitrella patens under blue- and red-light stimuli. / Miyazaki, Sho; Nakajima, Masatoshi; Kawaide, Hiroshi.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 35-43 (Methods in Molecular Biology; Vol. 1924).

Research output: Chapter in Book/Report/Conference proceedingChapter

Miyazaki, S, Nakajima, M & Kawaide, H 2019, Assays of protonemal growth responses in physcomitrella patens under blue- and red-light stimuli. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1924, Humana Press Inc., pp. 35-43. https://doi.org/10.1007/978-1-4939-9015-3_4
Miyazaki S, Nakajima M, Kawaide H. Assays of protonemal growth responses in physcomitrella patens under blue- and red-light stimuli. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 35-43. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9015-3_4
Miyazaki, Sho ; Nakajima, Masatoshi ; Kawaide, Hiroshi. / Assays of protonemal growth responses in physcomitrella patens under blue- and red-light stimuli. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 35-43 (Methods in Molecular Biology).
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