Assembly of actomyosin bundles in microfluidic channel

Shusei Kawara, Yuichi Hiratsuka, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Artificial reconstruction of a highly aligned actomyosin bundle utilizing a laminar-flow microfluidic device enables the fabrication of a bio-actuator mimicking skeletal muscle. At the boundary of two fluid streams involving fibrous myosinⅡ and actin macromolecules respectively, actomyosin bundles are spontaneously assembled and aligned along with the flow direction. We successfully obtained several millimeter-scale actomyosin bundles from the microfluidic devices and also revealed that the actin filament was highly aligned in the bundle, which could enhance the mechanical force produced by contraction. We conceive that our methodology for the assembly of actomyosin bundle would contribute to the development of bio-actuators and furthermore to the understanding of the self-organization mechanism and the mechanical characteristics.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages209-210
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period19/10/2719/10/31

Keywords

  • Actin
  • Actuator
  • Alignment
  • Microfluidics
  • Motor protein
  • MyosinⅡ

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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