Assembly of single adherent cells on mobile microplates

Hiroaki Onoe; Shoji Takeuchi

doi:10.1109/MEMSYS.2008.4443652

Assembly of single adherent cells on mobile microplates

Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

This paper describes single-cell-mounted mobile microplates. Microfabricated SiO₂ microplates (20-75 μm in diameter and 2 μm in thickness) carry single adherent cells. The microplates are released freely from a plate-holding PDMS substrate. These microplates enabled us to handle adherent cells as if they were floating cells. We applied this cell-handling technique to two types of assembly: (i) microplate-guided 2D assembly of heterogeneous cells for cell-cell interaction analysis, and (ii) 3D microplate assembly powered by cell traction force for fabricating 3D microstructures. These assembly experiments indicate that the cell-mounted mobile microplates make it possible to handle single adherent cells and have potentials to assemble cell-plate microstructures.

Original language	English
Title of host publication	MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems
Pages	300-303
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2008.4443652
Publication status	Published - 2008 Aug 29
Externally published	Yes
Event	21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson - Tucson, AZ, United States Duration: 2008 Jan 13 → 2008 Jan 17

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson
Country/Territory	United States
City	Tucson, AZ
Period	08/1/13 → 08/1/17

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2008.4443652

Cite this

Assembly of single adherent cells on mobile microplates. / Onoe, Hiroaki; Takeuchi, Shoji.

MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems. 2008. p. 300-303 4443652 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Onoe, H & Takeuchi, S 2008, Assembly of single adherent cells on mobile microplates. in MEMS 2008 Tucson - 21st IEEE International Conference on Micro Electro Mechanical Systems., 4443652, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 300-303, 21st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2008 Tucson, Tucson, AZ, United States, 08/1/13. https://doi.org/10.1109/MEMSYS.2008.4443652

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title = "Assembly of single adherent cells on mobile microplates",

abstract = "This paper describes single-cell-mounted mobile microplates. Microfabricated SiO2 microplates (20-75 μm in diameter and 2 μm in thickness) carry single adherent cells. The microplates are released freely from a plate-holding PDMS substrate. These microplates enabled us to handle adherent cells as if they were floating cells. We applied this cell-handling technique to two types of assembly: (i) microplate-guided 2D assembly of heterogeneous cells for cell-cell interaction analysis, and (ii) 3D microplate assembly powered by cell traction force for fabricating 3D microstructures. These assembly experiments indicate that the cell-mounted mobile microplates make it possible to handle single adherent cells and have potentials to assemble cell-plate microstructures.",

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doi = "10.1109/MEMSYS.2008.4443652",

language = "English",

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series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "300--303",

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AB - This paper describes single-cell-mounted mobile microplates. Microfabricated SiO2 microplates (20-75 μm in diameter and 2 μm in thickness) carry single adherent cells. The microplates are released freely from a plate-holding PDMS substrate. These microplates enabled us to handle adherent cells as if they were floating cells. We applied this cell-handling technique to two types of assembly: (i) microplate-guided 2D assembly of heterogeneous cells for cell-cell interaction analysis, and (ii) 3D microplate assembly powered by cell traction force for fabricating 3D microstructures. These assembly experiments indicate that the cell-mounted mobile microplates make it possible to handle single adherent cells and have potentials to assemble cell-plate microstructures.

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Assembly of single adherent cells on mobile microplates

Abstract

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