Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Vincent VanBuren, Yulan Piao, Dawood B. Dudekula, Yong Qian, Carter G. Carter, Patrick R. Martin, Carole A. Stagg, Uwem C. Bassey, Kazuhiro Aiba, Toshio Hamatani, George J. Kargul, Amber G. Luo, Janet Kelso, Winston Hide, Minoru S H Ko

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

Original languageEnglish
Pages (from-to)1999-2003
Number of pages5
JournalGenome Research
Volume12
Issue number12
DOIs
Publication statusPublished - 2002 Dec 1
Externally publishedYes

Fingerprint

Complementary DNA
Clone Cells
Databases
Gene Library
Libraries
Stem Cells
Embryonic Structures

ASJC Scopus subject areas

  • Genetics

Cite this

VanBuren, V., Piao, Y., Dudekula, D. B., Qian, Y., Carter, C. G., Martin, P. R., ... Ko, M. S. H. (2002). Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set. Genome Research, 12(12), 1999-2003. https://doi.org/10.1101/gr.633802

Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set. / VanBuren, Vincent; Piao, Yulan; Dudekula, Dawood B.; Qian, Yong; Carter, Carter G.; Martin, Patrick R.; Stagg, Carole A.; Bassey, Uwem C.; Aiba, Kazuhiro; Hamatani, Toshio; Kargul, George J.; Luo, Amber G.; Kelso, Janet; Hide, Winston; Ko, Minoru S H.

In: Genome Research, Vol. 12, No. 12, 01.12.2002, p. 1999-2003.

Research output: Contribution to journalArticle

VanBuren, V, Piao, Y, Dudekula, DB, Qian, Y, Carter, CG, Martin, PR, Stagg, CA, Bassey, UC, Aiba, K, Hamatani, T, Kargul, GJ, Luo, AG, Kelso, J, Hide, W & Ko, MSH 2002, 'Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set', Genome Research, vol. 12, no. 12, pp. 1999-2003. https://doi.org/10.1101/gr.633802
VanBuren V, Piao Y, Dudekula DB, Qian Y, Carter CG, Martin PR et al. Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set. Genome Research. 2002 Dec 1;12(12):1999-2003. https://doi.org/10.1101/gr.633802
VanBuren, Vincent ; Piao, Yulan ; Dudekula, Dawood B. ; Qian, Yong ; Carter, Carter G. ; Martin, Patrick R. ; Stagg, Carole A. ; Bassey, Uwem C. ; Aiba, Kazuhiro ; Hamatani, Toshio ; Kargul, George J. ; Luo, Amber G. ; Kelso, Janet ; Hide, Winston ; Ko, Minoru S H. / Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set. In: Genome Research. 2002 ; Vol. 12, No. 12. pp. 1999-2003.
@article{19d4d46b9ccd4822aec62ba8d3aec1a1,
title = "Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set",
abstract = "A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.",
author = "Vincent VanBuren and Yulan Piao and Dudekula, {Dawood B.} and Yong Qian and Carter, {Carter G.} and Martin, {Patrick R.} and Stagg, {Carole A.} and Bassey, {Uwem C.} and Kazuhiro Aiba and Toshio Hamatani and Kargul, {George J.} and Luo, {Amber G.} and Janet Kelso and Winston Hide and Ko, {Minoru S H}",
year = "2002",
month = "12",
day = "1",
doi = "10.1101/gr.633802",
language = "English",
volume = "12",
pages = "1999--2003",
journal = "Genome Research",
issn = "1088-9051",
publisher = "Cold Spring Harbor Laboratory Press",
number = "12",

}

TY - JOUR

T1 - Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

AU - VanBuren, Vincent

AU - Piao, Yulan

AU - Dudekula, Dawood B.

AU - Qian, Yong

AU - Carter, Carter G.

AU - Martin, Patrick R.

AU - Stagg, Carole A.

AU - Bassey, Uwem C.

AU - Aiba, Kazuhiro

AU - Hamatani, Toshio

AU - Kargul, George J.

AU - Luo, Amber G.

AU - Kelso, Janet

AU - Hide, Winston

AU - Ko, Minoru S H

PY - 2002/12/1

Y1 - 2002/12/1

N2 - A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

AB - A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

UR - http://www.scopus.com/inward/record.url?scp=0036907106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036907106&partnerID=8YFLogxK

U2 - 10.1101/gr.633802

DO - 10.1101/gr.633802

M3 - Article

VL - 12

SP - 1999

EP - 2003

JO - Genome Research

JF - Genome Research

SN - 1088-9051

IS - 12

ER -