Assessment of fixed charge density in regenerated cartilage by Gd-DTPA-enhanced MRI

Shogo Miyata, Kazuhiro Homma, Tomokazu Numano, Katsuko Furukawa, Tetsuya Tateishi, Takashi Ushida

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose: Applying regenerated cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of cartilage tissue. Magnetic resonance (MR) imaging of articular cartilage is well accepted and has been applied clinically in recent years. We attempt to establish a noninvasive method to evaluate the maturity of regenerated cartilage tissue using gadolinium-enhanced MR imaging. Methods: To reconstruct cartilaginous tissue, we embedded articular chondrocytes harvested from bovine humeral head in agarose gel and cultured the cells in vitro up to 4 weeks. The fixed charge density (FCD) of the cartilage was determined using MRI gadolinium exclusion method. The sulfated glycosaminoglycan (sGAG) content was determined by dimethylmethylene blue dye-binding assay. Results: The sGAG content and FCD of the regenerated cartilage increased with duration of culture. In the T1Gd maps, the [Gd-DTPA2-] in the specimen decreased, and the boundary between the sample disk and the bath solution of phosphate buffered saline (PBS) became clearer as time in culture increased. In the linear regression analysis, FCD and sGAG content correlated significantly. Conclusion: Gadolinium-enhanced MR imaging measurements can be useful predictors of the degree of cartilaginous tissue formation.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalMagnetic Resonance in Medical Sciences
Volume5
Issue number2
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

A73025
Gadolinium DTPA
Cartilage
Gadolinium
Magnetic Resonance Imaging
Humeral Head
Articular Cartilage
Chondrocytes
Baths
Sepharose
Cultured Cells
Linear Models
Coloring Agents
Joints
Gels
Phosphates
Regression Analysis

Keywords

  • Fixed charge density
  • Gd-DTPA
  • MRI
  • Regenerated cartilage
  • Sulfated glycosaminoglycan

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

Assessment of fixed charge density in regenerated cartilage by Gd-DTPA-enhanced MRI. / Miyata, Shogo; Homma, Kazuhiro; Numano, Tomokazu; Furukawa, Katsuko; Tateishi, Tetsuya; Ushida, Takashi.

In: Magnetic Resonance in Medical Sciences, Vol. 5, No. 2, 2006, p. 73-78.

Research output: Contribution to journalArticle

Miyata, Shogo ; Homma, Kazuhiro ; Numano, Tomokazu ; Furukawa, Katsuko ; Tateishi, Tetsuya ; Ushida, Takashi. / Assessment of fixed charge density in regenerated cartilage by Gd-DTPA-enhanced MRI. In: Magnetic Resonance in Medical Sciences. 2006 ; Vol. 5, No. 2. pp. 73-78.
@article{0309178a503a481f944b496357335d9b,
title = "Assessment of fixed charge density in regenerated cartilage by Gd-DTPA-enhanced MRI",
abstract = "Purpose: Applying regenerated cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of cartilage tissue. Magnetic resonance (MR) imaging of articular cartilage is well accepted and has been applied clinically in recent years. We attempt to establish a noninvasive method to evaluate the maturity of regenerated cartilage tissue using gadolinium-enhanced MR imaging. Methods: To reconstruct cartilaginous tissue, we embedded articular chondrocytes harvested from bovine humeral head in agarose gel and cultured the cells in vitro up to 4 weeks. The fixed charge density (FCD) of the cartilage was determined using MRI gadolinium exclusion method. The sulfated glycosaminoglycan (sGAG) content was determined by dimethylmethylene blue dye-binding assay. Results: The sGAG content and FCD of the regenerated cartilage increased with duration of culture. In the T1Gd maps, the [Gd-DTPA2-] in the specimen decreased, and the boundary between the sample disk and the bath solution of phosphate buffered saline (PBS) became clearer as time in culture increased. In the linear regression analysis, FCD and sGAG content correlated significantly. Conclusion: Gadolinium-enhanced MR imaging measurements can be useful predictors of the degree of cartilaginous tissue formation.",
keywords = "Fixed charge density, Gd-DTPA, MRI, Regenerated cartilage, Sulfated glycosaminoglycan",
author = "Shogo Miyata and Kazuhiro Homma and Tomokazu Numano and Katsuko Furukawa and Tetsuya Tateishi and Takashi Ushida",
year = "2006",
doi = "10.2463/mrms.5.73",
language = "English",
volume = "5",
pages = "73--78",
journal = "Magnetic Resonance in Medical Sciences",
issn = "1347-3182",
publisher = "Japan Society of Magnetic Resonance in Medicine",
number = "2",

}

TY - JOUR

T1 - Assessment of fixed charge density in regenerated cartilage by Gd-DTPA-enhanced MRI

AU - Miyata, Shogo

AU - Homma, Kazuhiro

AU - Numano, Tomokazu

AU - Furukawa, Katsuko

AU - Tateishi, Tetsuya

AU - Ushida, Takashi

PY - 2006

Y1 - 2006

N2 - Purpose: Applying regenerated cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of cartilage tissue. Magnetic resonance (MR) imaging of articular cartilage is well accepted and has been applied clinically in recent years. We attempt to establish a noninvasive method to evaluate the maturity of regenerated cartilage tissue using gadolinium-enhanced MR imaging. Methods: To reconstruct cartilaginous tissue, we embedded articular chondrocytes harvested from bovine humeral head in agarose gel and cultured the cells in vitro up to 4 weeks. The fixed charge density (FCD) of the cartilage was determined using MRI gadolinium exclusion method. The sulfated glycosaminoglycan (sGAG) content was determined by dimethylmethylene blue dye-binding assay. Results: The sGAG content and FCD of the regenerated cartilage increased with duration of culture. In the T1Gd maps, the [Gd-DTPA2-] in the specimen decreased, and the boundary between the sample disk and the bath solution of phosphate buffered saline (PBS) became clearer as time in culture increased. In the linear regression analysis, FCD and sGAG content correlated significantly. Conclusion: Gadolinium-enhanced MR imaging measurements can be useful predictors of the degree of cartilaginous tissue formation.

AB - Purpose: Applying regenerated cartilage in a clinical setting requires noninvasive evaluation to detect the maturity of cartilage tissue. Magnetic resonance (MR) imaging of articular cartilage is well accepted and has been applied clinically in recent years. We attempt to establish a noninvasive method to evaluate the maturity of regenerated cartilage tissue using gadolinium-enhanced MR imaging. Methods: To reconstruct cartilaginous tissue, we embedded articular chondrocytes harvested from bovine humeral head in agarose gel and cultured the cells in vitro up to 4 weeks. The fixed charge density (FCD) of the cartilage was determined using MRI gadolinium exclusion method. The sulfated glycosaminoglycan (sGAG) content was determined by dimethylmethylene blue dye-binding assay. Results: The sGAG content and FCD of the regenerated cartilage increased with duration of culture. In the T1Gd maps, the [Gd-DTPA2-] in the specimen decreased, and the boundary between the sample disk and the bath solution of phosphate buffered saline (PBS) became clearer as time in culture increased. In the linear regression analysis, FCD and sGAG content correlated significantly. Conclusion: Gadolinium-enhanced MR imaging measurements can be useful predictors of the degree of cartilaginous tissue formation.

KW - Fixed charge density

KW - Gd-DTPA

KW - MRI

KW - Regenerated cartilage

KW - Sulfated glycosaminoglycan

UR - http://www.scopus.com/inward/record.url?scp=34548510550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548510550&partnerID=8YFLogxK

U2 - 10.2463/mrms.5.73

DO - 10.2463/mrms.5.73

M3 - Article

C2 - 17008763

AN - SCOPUS:34548510550

VL - 5

SP - 73

EP - 78

JO - Magnetic Resonance in Medical Sciences

JF - Magnetic Resonance in Medical Sciences

SN - 1347-3182

IS - 2

ER -