Assessment of mitophagy in iPS cell-derived neurons

Kei Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, Wado Akamatsu

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Aberrant mitochondrial function is associated with many neurological diseases. Mitophagy is a key mechanism for the elimination of damaged mitochondria and maintenance of mitochondrial homeostasis. Induced pluripotent stem (iPS) cell technologies developed over the last decade have allowed us to analyze functions of the human neuron. Here we describe an efficient induction method from human iPS cells to neurons, followed by an image-based mitophagy assay.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages59-67
Number of pages9
DOIs
Publication statusPublished - 2018 Jan 1

Publication series

NameMethods in Molecular Biology
Volume1759
ISSN (Print)1064-3745

Fingerprint

Mitochondrial Degradation
Induced Pluripotent Stem Cells
Neurons
Mitochondria
Homeostasis
Maintenance
Technology

Keywords

  • Autophagy
  • iPS cells
  • Mitophagy
  • Neurons
  • Parkinson’s disease

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ishikawa, K. I., Yamaguchi, A., Okano, H., & Akamatsu, W. (2018). Assessment of mitophagy in iPS cell-derived neurons. In Methods in Molecular Biology (pp. 59-67). (Methods in Molecular Biology; Vol. 1759). Humana Press Inc.. https://doi.org/10.1007/7651_2017_10

Assessment of mitophagy in iPS cell-derived neurons. / Ishikawa, Kei Ichi; Yamaguchi, Akihiro; Okano, Hideyuki; Akamatsu, Wado.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 59-67 (Methods in Molecular Biology; Vol. 1759).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ishikawa, KI, Yamaguchi, A, Okano, H & Akamatsu, W 2018, Assessment of mitophagy in iPS cell-derived neurons. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1759, Humana Press Inc., pp. 59-67. https://doi.org/10.1007/7651_2017_10
Ishikawa KI, Yamaguchi A, Okano H, Akamatsu W. Assessment of mitophagy in iPS cell-derived neurons. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 59-67. (Methods in Molecular Biology). https://doi.org/10.1007/7651_2017_10
Ishikawa, Kei Ichi ; Yamaguchi, Akihiro ; Okano, Hideyuki ; Akamatsu, Wado. / Assessment of mitophagy in iPS cell-derived neurons. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 59-67 (Methods in Molecular Biology).
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