TY - JOUR
T1 - Assessment of real-time PCR for diagnosis of Mycoplasma pneumoniae pneumonia in pediatric patients
AU - Morozumi, Miyuki
AU - Ito, Akira
AU - Murayama, Somay Y.
AU - Hasegawa, Keiko
AU - Kobayashi, Reiko
AU - Iwata, Satoshi
AU - Kawamura, Naohisa
AU - Kuroki, Haruo
AU - Nakayama, Eiichi
AU - Tajima, Takeshi
AU - Ubukata, Kimiko
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/2
Y1 - 2006/2
N2 - We developed a real-time PCR to detect Mycoplasma pneumoniae with a primer set designed for the 16S rRNA gene. Clinical samples (n = 937) were collected from children with community-acquired pneumonia between April 2002 and March 2004 at 12 Japanese medical institutions. Sensitivity of real-time PCR was calculated as 10 colony-forming units per reaction tube using a pMP01 plasmid carrying a 225-bp target DNA fragment of the 16S rRNA gene in M. pneumoniae M129, a standard strain. Results, obtained within 2 h, were compared with those of conventional culture and serologic methods. Of all cases tested, 151 (16.4%) and 129 (13.8%) were positive for M. pneumoniae by real-time PCR and by culture, respectively. Among the 151 cases, almost all of those tested serologically by passive agglutination showed a rise in M. pneumoniae antibody titre between acute and convalescent sera. We conclude that this real-time PCR can identify M. pneumoniae rapidly and fulfills the need for rapid identification, high sensitivity, and high specificity.
AB - We developed a real-time PCR to detect Mycoplasma pneumoniae with a primer set designed for the 16S rRNA gene. Clinical samples (n = 937) were collected from children with community-acquired pneumonia between April 2002 and March 2004 at 12 Japanese medical institutions. Sensitivity of real-time PCR was calculated as 10 colony-forming units per reaction tube using a pMP01 plasmid carrying a 225-bp target DNA fragment of the 16S rRNA gene in M. pneumoniae M129, a standard strain. Results, obtained within 2 h, were compared with those of conventional culture and serologic methods. Of all cases tested, 151 (16.4%) and 129 (13.8%) were positive for M. pneumoniae by real-time PCR and by culture, respectively. Among the 151 cases, almost all of those tested serologically by passive agglutination showed a rise in M. pneumoniae antibody titre between acute and convalescent sera. We conclude that this real-time PCR can identify M. pneumoniae rapidly and fulfills the need for rapid identification, high sensitivity, and high specificity.
KW - Community-acquired pneumonia
KW - Mycoplasma pneumoniae culture
KW - Mycoplasma pneumoniae identification
KW - Pediatrics
KW - Real-time PCR
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U2 - 10.1139/w05-118
DO - 10.1139/w05-118
M3 - Article
C2 - 16541148
AN - SCOPUS:33744474048
VL - 52
SP - 125
EP - 129
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
SN - 0008-4166
IS - 2
ER -