Assessment of real-time PCR for diagnosis of Mycoplasma pneumoniae pneumonia in pediatric patients

Miyuki Morozumi, Akira Ito, Somay Y. Murayama, Keiko Hasegawa, Reiko Kobayashi, Satoshi Iwata, Naohisa Kawamura, Haruo Kuroki, Eiichi Nakayama, Takeshi Tajima, Kimiko Ubukata

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28 Citations (Scopus)

Abstract

We developed a real-time PCR to detect Mycoplasma pneumoniae with a primer set designed for the 16S rRNA gene. Clinical samples (n = 937) were collected from children with community-acquired pneumonia between April 2002 and March 2004 at 12 Japanese medical institutions. Sensitivity of real-time PCR was calculated as 10 colony-forming units per reaction tube using a pMP01 plasmid carrying a 225-bp target DNA fragment of the 16S rRNA gene in M. pneumoniae M129, a standard strain. Results, obtained within 2 h, were compared with those of conventional culture and serologic methods. Of all cases tested, 151 (16.4%) and 129 (13.8%) were positive for M. pneumoniae by real-time PCR and by culture, respectively. Among the 151 cases, almost all of those tested serologically by passive agglutination showed a rise in M. pneumoniae antibody titre between acute and convalescent sera. We conclude that this real-time PCR can identify M. pneumoniae rapidly and fulfills the need for rapid identification, high sensitivity, and high specificity.

Original languageEnglish
Pages (from-to)125-129
Number of pages5
JournalCanadian Journal of Microbiology
Volume52
Issue number2
DOIs
Publication statusPublished - 2006 Feb

Keywords

  • Community-acquired pneumonia
  • Mycoplasma pneumoniae culture
  • Mycoplasma pneumoniae identification
  • Pediatrics
  • Real-time PCR

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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  • Cite this

    Morozumi, M., Ito, A., Murayama, S. Y., Hasegawa, K., Kobayashi, R., Iwata, S., Kawamura, N., Kuroki, H., Nakayama, E., Tajima, T., & Ubukata, K. (2006). Assessment of real-time PCR for diagnosis of Mycoplasma pneumoniae pneumonia in pediatric patients. Canadian Journal of Microbiology, 52(2), 125-129. https://doi.org/10.1139/w05-118