Abstract
We developed a real-time PCR to detect Mycoplasma pneumoniae with a primer set designed for the 16S rRNA gene. Clinical samples (n = 937) were collected from children with community-acquired pneumonia between April 2002 and March 2004 at 12 Japanese medical institutions. Sensitivity of real-time PCR was calculated as 10 colony-forming units per reaction tube using a pMP01 plasmid carrying a 225-bp target DNA fragment of the 16S rRNA gene in M. pneumoniae M129, a standard strain. Results, obtained within 2 h, were compared with those of conventional culture and serologic methods. Of all cases tested, 151 (16.4%) and 129 (13.8%) were positive for M. pneumoniae by real-time PCR and by culture, respectively. Among the 151 cases, almost all of those tested serologically by passive agglutination showed a rise in M. pneumoniae antibody titre between acute and convalescent sera. We conclude that this real-time PCR can identify M. pneumoniae rapidly and fulfills the need for rapid identification, high sensitivity, and high specificity.
| Original language | English |
|---|---|
| Pages (from-to) | 125-129 |
| Number of pages | 5 |
| Journal | Canadian Journal of Microbiology |
| Volume | 52 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2006 Feb |
| Externally published | Yes |
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Keywords
- Community-acquired pneumonia
- Mycoplasma pneumoniae culture
- Mycoplasma pneumoniae identification
- Pediatrics
- Real-time PCR
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Immunology
- Microbiology
Cite this
Assessment of real-time PCR for diagnosis of Mycoplasma pneumoniae pneumonia in pediatric patients. / Morozumi, Miyuki; Ito, Akira; Murayama, Somay Y.; Hasegawa, Keiko; Kobayashi, Reiko; Iwata, Satoshi; Kawamura, Naohisa; Kuroki, Haruo; Nakayama, Eiichi; Tajima, Takeshi; Ubukata, Kimiko.
In: Canadian Journal of Microbiology, Vol. 52, No. 2, 02.2006, p. 125-129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assessment of real-time PCR for diagnosis of Mycoplasma pneumoniae pneumonia in pediatric patients
AU - Morozumi, Miyuki
AU - Ito, Akira
AU - Murayama, Somay Y.
AU - Hasegawa, Keiko
AU - Kobayashi, Reiko
AU - Iwata, Satoshi
AU - Kawamura, Naohisa
AU - Kuroki, Haruo
AU - Nakayama, Eiichi
AU - Tajima, Takeshi
AU - Ubukata, Kimiko
PY - 2006/2
Y1 - 2006/2
N2 - We developed a real-time PCR to detect Mycoplasma pneumoniae with a primer set designed for the 16S rRNA gene. Clinical samples (n = 937) were collected from children with community-acquired pneumonia between April 2002 and March 2004 at 12 Japanese medical institutions. Sensitivity of real-time PCR was calculated as 10 colony-forming units per reaction tube using a pMP01 plasmid carrying a 225-bp target DNA fragment of the 16S rRNA gene in M. pneumoniae M129, a standard strain. Results, obtained within 2 h, were compared with those of conventional culture and serologic methods. Of all cases tested, 151 (16.4%) and 129 (13.8%) were positive for M. pneumoniae by real-time PCR and by culture, respectively. Among the 151 cases, almost all of those tested serologically by passive agglutination showed a rise in M. pneumoniae antibody titre between acute and convalescent sera. We conclude that this real-time PCR can identify M. pneumoniae rapidly and fulfills the need for rapid identification, high sensitivity, and high specificity.
AB - We developed a real-time PCR to detect Mycoplasma pneumoniae with a primer set designed for the 16S rRNA gene. Clinical samples (n = 937) were collected from children with community-acquired pneumonia between April 2002 and March 2004 at 12 Japanese medical institutions. Sensitivity of real-time PCR was calculated as 10 colony-forming units per reaction tube using a pMP01 plasmid carrying a 225-bp target DNA fragment of the 16S rRNA gene in M. pneumoniae M129, a standard strain. Results, obtained within 2 h, were compared with those of conventional culture and serologic methods. Of all cases tested, 151 (16.4%) and 129 (13.8%) were positive for M. pneumoniae by real-time PCR and by culture, respectively. Among the 151 cases, almost all of those tested serologically by passive agglutination showed a rise in M. pneumoniae antibody titre between acute and convalescent sera. We conclude that this real-time PCR can identify M. pneumoniae rapidly and fulfills the need for rapid identification, high sensitivity, and high specificity.
KW - Community-acquired pneumonia
KW - Mycoplasma pneumoniae culture
KW - Mycoplasma pneumoniae identification
KW - Pediatrics
KW - Real-time PCR
UR - http://www.scopus.com/inward/record.url?scp=33744474048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744474048&partnerID=8YFLogxK
U2 - 10.1139/w05-118
DO - 10.1139/w05-118
M3 - Article
C2 - 16541148
AN - SCOPUS:33744474048
VL - 52
SP - 125
EP - 129
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
SN - 0008-4166
IS - 2
ER -