Assignment of the myeloperoxidase gene MPO to human chromosome 17 using somatic cell hybrids and flow-sorted chromosomes

Jun Kudo, Shinsei Minoshima, Kazuya Hashinaka, Chika Nishio, Michiyuki Yamada, Yoshiko Shimizu, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A cDNA coding for human myeloperoxidase (MPO) was used as a probe to study MPO gene structure and to determine the chromosomal location of the gene in the human genome. Southern blot hybridization of restriction endonuclease digests of human DNA with the MPO cDNA probe showed that a single gene for human MPO was present in the human genome. Southern blot hybridization experiments with human-mouse cell hybrid DNAs containing various subsets of human chromosomes revealed that the human MPO gene is located on chromosome 17. This conclusion was supported by DNA spot-blot hybridization using flow-sorted human chromosomes.

Original languageEnglish
Pages (from-to)315-324
Number of pages10
JournalThe Japanese Journal of Human Genetics
Volume33
Issue number3
DOIs
Publication statusPublished - 1988 Sep

Fingerprint

Chromosomes, Human, Pair 17
Hybrid Cells
Human Chromosomes
Peroxidase
Chromosomes
Genes
Human Genome
Southern Blotting
DNA
Complementary DNA
DNA Restriction Enzymes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Assignment of the myeloperoxidase gene MPO to human chromosome 17 using somatic cell hybrids and flow-sorted chromosomes. / Kudo, Jun; Minoshima, Shinsei; Hashinaka, Kazuya; Nishio, Chika; Yamada, Michiyuki; Shimizu, Yoshiko; Shimizu, Nobuyoshi.

In: The Japanese Journal of Human Genetics, Vol. 33, No. 3, 09.1988, p. 315-324.

Research output: Contribution to journalArticle

Kudo, Jun ; Minoshima, Shinsei ; Hashinaka, Kazuya ; Nishio, Chika ; Yamada, Michiyuki ; Shimizu, Yoshiko ; Shimizu, Nobuyoshi. / Assignment of the myeloperoxidase gene MPO to human chromosome 17 using somatic cell hybrids and flow-sorted chromosomes. In: The Japanese Journal of Human Genetics. 1988 ; Vol. 33, No. 3. pp. 315-324.
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