Association between G2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes

Marco Durante, Yoshiya Furusawa, Hideyuki Majima, Testsuya Kawata, Eisuke Gotoh

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We have studied the induction of chromosomal aberrations in human lymphocytes exposed in Go to X rays or carbon ions. Aberrations were analyzed in G0, G1, G2 or M phase. Analysis during the interphase was performed by chemically induced premature chromosome condensation, which allows scoring of aberrations in G1, G2 and M phase; fusion-induced premature chromosome condensation was used to analyze the damage in Go cells after incubation for repair; M-phase cells were obtained by conventional Colcemid block. Aberrations were scored by Giemsa staining or fluorescence in situ hybridization (chromosomes 2 and 4). Similar yields of fragments were observed in G1 and G2 phase, but lower yields were scored in metaphase. The frequency of chromosomal exchanges was similar in G0 (after repair), G2 and M phase for cells exposed to X rays, while a lower frequency of exchanges was observed in M phase when lymphocytes were irradiated with high-LET carbon ions. The results suggest that radiation-induced G2-phase block is associated with unrejoined chromosome fragments induced by radiation exposure during G0.

Original languageEnglish
Pages (from-to)670-676
Number of pages7
JournalRadiation Research
Volume151
Issue number6
DOIs
Publication statusPublished - 1999 Jun
Externally publishedYes

Fingerprint

G2 Phase
lymphocytes
chromosomes
interphase
Cell Division
aberration
Chromosomes
fragments
Lymphocytes
Radiation
G1 Phase
radiation
mitosis
condensation
cells
Carbon
scoring
X-Rays
carbon
Demecolcine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Association between G2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes. / Durante, Marco; Furusawa, Yoshiya; Majima, Hideyuki; Kawata, Testsuya; Gotoh, Eisuke.

In: Radiation Research, Vol. 151, No. 6, 06.1999, p. 670-676.

Research output: Contribution to journalArticle

Durante, Marco ; Furusawa, Yoshiya ; Majima, Hideyuki ; Kawata, Testsuya ; Gotoh, Eisuke. / Association between G2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes. In: Radiation Research. 1999 ; Vol. 151, No. 6. pp. 670-676.
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