Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men

Mariko Naito, Koichi Miyaki, Toru Naito, Ling Zhang, Keika Hoshi, Asako Hara, Katsunori Masaki, Shugo Tohyama, Masaaki Muramatsu, Nobuyuki Hamajima, Takeo Nakayama

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: The vitamin D receptor (VDR) is involved in a variety of biological processes, such as bone metabolism and modulation of the immune response. Recent findings suggest that the pathway involving bone mineral density-mediated effects is important for the development of periodontitis, but their effects of combined VDR gene polymorphisms have not been confirmed on periodontitis. We assessed the relationship between ApaI, BsmI, and FokI VDR polymorphisms and the risk of severe chronic periodontitis among Japanese adult men. Materials and Methods: In a cross-sectional study, we examined 97 unrelated healthy Japanese men (mean age: 45.6 years, range: 22-59). A clinical examination was performed at a worksite health checkup, and information was obtained using a self-reported questionnaire. DNA was extracted from whole blood, and the VDR ApaI, BsmI, and FokI polymorphisms were genotyped using polymerase chain reaction. Results: F-carriers of FokI VDR polymorphisms were less likely to develop severe chronic periodontitis than non-F-carriers (p = 0.09). The ApaI and BsmI VDR polymorphisms did not show significant differences in the alleles or genotypes between the subjects with or without severe chronic periodontitis. The haplotype analysis of the three combined VDR polymorphisms revealed that the Abf homozygote had a notably higher prevalence of severe chronic periodontitis than the others, and adjustments for age, smoking status, number of teeth present, and prevalence of diabetes did not change this association (OR = 7.5; 95% CI = 1.6-34.4; p = 0.01). Conclusion: The VDR haplotype constructed from the ApaI, BsmI, and FokI polymorphisms is related to the risk of severe chronic periodontitis in Japanese men.

Original languageEnglish
Pages (from-to)216-222
Number of pages7
JournalInternational Journal of Medical Sciences
Volume4
Issue number4
DOIs
Publication statusPublished - 2007 Aug 22

Fingerprint

Chronic Periodontitis
Calcitriol Receptors
Haplotypes
Genes
Periodontitis
Biological Phenomena
Homozygote
Workplace
Bone Density
Tooth
Cross-Sectional Studies
Smoking
Alleles
Genotype
Bone and Bones
Polymerase Chain Reaction
DNA
Health

Keywords

  • Chronic periodontitis
  • Haplotypes
  • Polymorphism
  • Vitamin D receptor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men. / Naito, Mariko; Miyaki, Koichi; Naito, Toru; Zhang, Ling; Hoshi, Keika; Hara, Asako; Masaki, Katsunori; Tohyama, Shugo; Muramatsu, Masaaki; Hamajima, Nobuyuki; Nakayama, Takeo.

In: International Journal of Medical Sciences, Vol. 4, No. 4, 22.08.2007, p. 216-222.

Research output: Contribution to journalArticle

Naito, M, Miyaki, K, Naito, T, Zhang, L, Hoshi, K, Hara, A, Masaki, K, Tohyama, S, Muramatsu, M, Hamajima, N & Nakayama, T 2007, 'Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men', International Journal of Medical Sciences, vol. 4, no. 4, pp. 216-222. https://doi.org/10.7150/ijms.4.216
Naito, Mariko ; Miyaki, Koichi ; Naito, Toru ; Zhang, Ling ; Hoshi, Keika ; Hara, Asako ; Masaki, Katsunori ; Tohyama, Shugo ; Muramatsu, Masaaki ; Hamajima, Nobuyuki ; Nakayama, Takeo. / Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men. In: International Journal of Medical Sciences. 2007 ; Vol. 4, No. 4. pp. 216-222.
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AU - Naito, Mariko

AU - Miyaki, Koichi

AU - Naito, Toru

AU - Zhang, Ling

AU - Hoshi, Keika

AU - Hara, Asako

AU - Masaki, Katsunori

AU - Tohyama, Shugo

AU - Muramatsu, Masaaki

AU - Hamajima, Nobuyuki

AU - Nakayama, Takeo

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N2 - Background: The vitamin D receptor (VDR) is involved in a variety of biological processes, such as bone metabolism and modulation of the immune response. Recent findings suggest that the pathway involving bone mineral density-mediated effects is important for the development of periodontitis, but their effects of combined VDR gene polymorphisms have not been confirmed on periodontitis. We assessed the relationship between ApaI, BsmI, and FokI VDR polymorphisms and the risk of severe chronic periodontitis among Japanese adult men. Materials and Methods: In a cross-sectional study, we examined 97 unrelated healthy Japanese men (mean age: 45.6 years, range: 22-59). A clinical examination was performed at a worksite health checkup, and information was obtained using a self-reported questionnaire. DNA was extracted from whole blood, and the VDR ApaI, BsmI, and FokI polymorphisms were genotyped using polymerase chain reaction. Results: F-carriers of FokI VDR polymorphisms were less likely to develop severe chronic periodontitis than non-F-carriers (p = 0.09). The ApaI and BsmI VDR polymorphisms did not show significant differences in the alleles or genotypes between the subjects with or without severe chronic periodontitis. The haplotype analysis of the three combined VDR polymorphisms revealed that the Abf homozygote had a notably higher prevalence of severe chronic periodontitis than the others, and adjustments for age, smoking status, number of teeth present, and prevalence of diabetes did not change this association (OR = 7.5; 95% CI = 1.6-34.4; p = 0.01). Conclusion: The VDR haplotype constructed from the ApaI, BsmI, and FokI polymorphisms is related to the risk of severe chronic periodontitis in Japanese men.

AB - Background: The vitamin D receptor (VDR) is involved in a variety of biological processes, such as bone metabolism and modulation of the immune response. Recent findings suggest that the pathway involving bone mineral density-mediated effects is important for the development of periodontitis, but their effects of combined VDR gene polymorphisms have not been confirmed on periodontitis. We assessed the relationship between ApaI, BsmI, and FokI VDR polymorphisms and the risk of severe chronic periodontitis among Japanese adult men. Materials and Methods: In a cross-sectional study, we examined 97 unrelated healthy Japanese men (mean age: 45.6 years, range: 22-59). A clinical examination was performed at a worksite health checkup, and information was obtained using a self-reported questionnaire. DNA was extracted from whole blood, and the VDR ApaI, BsmI, and FokI polymorphisms were genotyped using polymerase chain reaction. Results: F-carriers of FokI VDR polymorphisms were less likely to develop severe chronic periodontitis than non-F-carriers (p = 0.09). The ApaI and BsmI VDR polymorphisms did not show significant differences in the alleles or genotypes between the subjects with or without severe chronic periodontitis. The haplotype analysis of the three combined VDR polymorphisms revealed that the Abf homozygote had a notably higher prevalence of severe chronic periodontitis than the others, and adjustments for age, smoking status, number of teeth present, and prevalence of diabetes did not change this association (OR = 7.5; 95% CI = 1.6-34.4; p = 0.01). Conclusion: The VDR haplotype constructed from the ApaI, BsmI, and FokI polymorphisms is related to the risk of severe chronic periodontitis in Japanese men.

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